Antimicrobial and antibiofilm capacity of chitosan nanoparticles against wild type strain of pseudomonas sp. Isolated from milk of cows diagnosed with bovine mastitis
Autor
Aguayo, Paula Rivera
Larenas, Tamara Bruna
Godoy, Carlos Alarcón
Rivas, Bernardita Cayupe
González-Casanova, Jorge Enrique
Rojas-Gómez, Diana Marcela
Fuentes, Nelson Caro
Resumen
Bovine mastitis (BM) is the most prevalent bacterial infection in the livestock sector, affecting the dairy industry greatly. The prevention and treatment of this disease is mainly made via antibiotics, but the increasing antimicrobial resistance of pathogens has affected the efficiency of conventional drugs. Pseudomonas sp. is one of the pathogens involved in this infection. The therapeutic rate of cure for this environmental mastitis-causing pathogen is practically zero, regardless of treatment. Biofilm formation has been one of the main virulence mechanisms of Pseudomonas hence presenting resistance to antibiotic therapy. We have manufactured chitosan nanoparticles (NQo) with tripolyphosphate (TPP) using ionotropic gelation. These NQo were confronted against a Pseudomonas sp. strain isolated from milk samples of cows diagnosed with BM, to evaluate their antimicrobial and antibiofilm capacity. The NQo showed great antibacterial effect in the minimum inhibitory concentrations (MIC), minimum bactericidal concentration (MBC) and disk diffusion assays. Using sub lethal concentrations, NQo were tested for inhibition of biofilm formation. The results show that the nanoparticles exhibited biofilm inhibition and were capable of eradicate pre-existing mature biofilm. These findings indicate that the NQo could act as a potential alternative to antibiotic treatment of BM.
Colecciones
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Article
Nanomaterials for Potential Detection and Remediation: A Review of Their Analytical and Environmental Applications (2024)
Salazar Sandoval, Sebastián; Bruna, Tamara; Maldonado-Bravo, Francisca; Jara, Paul; Caro, Nelson; Rojas-Romo, Carlos; González-Casanova, Jorge; Gómez, Diana Rojas; Yutronic, Nicolás; Urzúa, Marcela; ... (Multidisciplinary Digital Publishing Institute (MDPI), 2023)The rapid increase in industrialization and human population is leading to critical levels of environmental pollutants, such as agrochemicals or heavy metals, which affect the preservation and integrity of ecosystems, the ... -
Article
New Evidence on BPA’s Role in Adipose Tissue Development of Proinflammatory Processes and Its Relationship with Obesity (2024)
González-Casanova, Jorge Enrique; Bermúdez, Valmore; Caro Fuentes, Nelson Javier; Angarita, Lissé Chiquinquirá; Caicedo, Nelson Hernando; Rivas Muñoz, Jocelyn; Rojas-Gómez, Diana Marcela (Multidisciplinary Digital Publishing Institute (MDPI), 2023)Bisphenol A (BPA) is a xenobiotic with endocrine disruptor properties which interacts with various receptors, eliciting a cellular response. In the plastic industry, BPA is widely used in the production of polycarbonate ... -
Article
New insights on the role of connexins and gap junctions channels in adipose tissue and obesity (2021)
González-Casanova, Jorge Enrique; Durán-Agüero, Samuel; Caro-Fuentes, Nelson Javier; Gamboa-Arancibia, Maria Elena; Bruna, Tamara C.; Bermúdez, Valmore; Rojas Gómez, Diana (MDPI, 2021-11-02)Due to the inability to curb the excessive increase in the prevalence of obesity and over-weight, it is necessary to comprehend in more detail the factors involved in the pathophysiology and to appreciate more clearly the ...