New nanocomposite made of cashew apple bagasse lignin and Fe3O4 for immobilizing of lipase B from Candida antarctica aiming at esterification
Autor
Serpa, Juliana De França
Matias, Gabriel A.B.
Almeida Fechine, Pierre Basílio
da Costa, Victor M.
Melo Freire, Rafael
Casagrande Denardin, Juliano
Barros Gonçalves, Luciana Rocha
Casimiro De Macedo, André
Valderez Ponte Rocha, Maria
Resumen
BACKGROUND: Novel magnetite-lignin hybrid materials were synthesized using lignin from cashew apple bagasse (CAB) and iron oxide nanoparticles (magnetite, Fe3O4). The new material, termed MNs/Lig (magnetite conjugated with lignin), was used as a support for the immobilization of lipase B from Candida antarctica (CAL─B), aiming to obtain an active and stable biocatalyst that is easily recoverable from the reactional medium. RESULTS: The magnetite/lignin composite showed a higher thermal stability than pure lignin, and it presented good magnetic properties, both before and after the immobilization procedure. The immobilization yield was higher using the unaltered MN/Lig as a support than when using the glutaraldehyde-activated MNs/Lig (MNs/Lig_GA), with yields of 66.6% and 40.0%, respectively. The presence of Triton X-100 did not influence the immobilization yield or the thermal stability of the biocatalyst when employing the unaltered MNs/Lig. However, it influenced the thermal stability of the lipase immobilized on MNs/Lig_GA, lowering the biocatalyst stability, which showed a half-life 11-fold lower than that of MNs/Lig_GA_CALB. The biocatalysts were tested with regards to the synthesis of ethyl oleate using oleic acid and ethanol as substrates, and 2-ethylhexyl oleate (a biolubricant) using oleic acid and 2-ethyl-hexanol as substrate. The conversions obtained by MNs/Lig_Tri_CALB were 88.2% and 76.7% for ethyl oleate and 2-ethylhexyl oleate, respectively. CONCLUSION: The novel strategy presented in this work for obtaining a biocatalyst using synthesized hybrids (MNs/Lig) may be a promising route for lipase immobilization because it can be considered environmentally benign and it shows a strong potential use in several reactions of industrial interest.
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