Analysis of the Zonula occludens Toxin Found in the Genome of the Chilean Non-toxigenic Vibrio parahaemolyticus Strain PMC53.7
Autor
Pérez-Reytor, Diliana Celeste
Pavón, Alequis
López-Joven, Carmen
Ramírez-Araya, Sebastián
Peña-Varas, Carlos
Plaza, Nicolás
Alegría-Arcos, Melissa
Corsini, Gino
Jaña, Víctor
Pavez, Leonardo
del Pozo, Talía
Bastías, Roberto
Blondel, Carlos J.
Ramírez, David
García, Katherine P.
Resumen
Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly described in Vibrio cholerae, is a secreted toxin that increases intestinal permeability. Recently, we identified Zot-encoding genes in the genomes of highly cytotoxic Chilean V. parahaemolyticus strains, including the non-toxigenic clinical strain PMC53.7. To gain insights into a possible role of Zot in V. parahaemolyticus, we analyzed whether it could be responsible for cytotoxicity. However, we observed a barely positive correlation between Caco-2 cell membrane damage and Zot mRNA expression during PMC53.7 infection and non-cytotoxicity induction in response to purified PMC53.7-Zot. Unusually, we observed a particular actin disturbance on cells infected with PMC53.7. Based on this observation, we decided to compare the sequence of PMC53.7-Zot with Zot of human pathogenic species such as V. cholerae, Campylobacter concisus, Neisseria meningitidis, and other V. parahaemolyticus strains, using computational tools. The PMC53.7-Zot was compared with other toxins and identified as an endotoxin with conserved motifs in the N-terminus and a variable C-terminal region and without FCIGRL peptide. Notably, the C-terminal diversity among Zots meant that not all of them could be identified as toxins. Structurally, PMC53.7-Zot was modeled as a transmembrane protein. Our results suggested that it has partial 3D structure similarity with V. cholerae-Zot. Probably, the PMC53.7-Zot would affect the actin cytoskeletal, but, in the absence of FCIGRL, the mechanisms of actions must be elucidated.
Colecciones
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Article
Conservation of small regulatory RNAs in Vibrio parahaemolyticus: Possible role of RNA-OUT encoded by the pathogenicity island (VPaI-7) of pandemic strains (2020)
Plaza N.; Pérez-Reytor D.; Ramírez-Araya S.; Pavón A.; Corsini G.; Loyola D.E.; Jaña V.; Pavéz L.; Navarrete P.; Bastías R.; ... (MDPI AG, 2019) -
Article
Presence of Zonula Occludens Toxin-Coding Genes among Vibrio parahaemolyticus Isolates of Clinical and Environmental Origin (2024)
Iribarren, Cristian; Plaza, Nicolás; Ramírez-Araya, Sebastián; Pérez-Reytor, Diliana; Urrutia, Ítalo M.; Suffredini, Elisabetta; Vicenza, Teresa; Ulloa, Soledad; Fernández, Jorge; Navarrete, Paola; ... (Multidisciplinary Digital Publishing Institute (MDPI), 2024)In recent studies, emphasis has been placed on the zonula occludens toxin (Zot) from the non-toxigenic Vibrio parahaemolyticus strain PMC53.7 as an agent inducing alterations in the actin cytoskeleton of infected Caco-2 ... -
Article
Exploring the genomic traits of non-toxigenic Vibrio parahaemolyticus strains isolated in southern Chile (2020)
Castillo D.; Pérez-Reytor D.; Plaza N.; Ramírez-Araya S.; Blondel C.J.; Corsini G.; Bastías R.; Loyola D.E.; Jaña V.; Pavez L.; ... (Frontiers Media S.A., 2018)