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Analysis of the Zonula occludens Toxin Found in the Genome of the Chilean Non-toxigenic Vibrio parahaemolyticus Strain PMC53.7
dc.contributor.author | Pérez-Reytor, Diliana Celeste | |
dc.contributor.author | Pavón, Alequis | |
dc.contributor.author | López-Joven, Carmen | |
dc.contributor.author | Ramírez-Araya, Sebastián | |
dc.contributor.author | Peña-Varas, Carlos | |
dc.contributor.author | Plaza, Nicolás | |
dc.contributor.author | Alegría-Arcos, Melissa | |
dc.contributor.author | Corsini, Gino | |
dc.contributor.author | Jaña, Víctor | |
dc.contributor.author | Pavez, Leonardo | |
dc.contributor.author | del Pozo, Talía | |
dc.contributor.author | Bastías, Roberto | |
dc.contributor.author | Blondel, Carlos J. | |
dc.contributor.author | Ramírez, David | |
dc.contributor.author | García, Katherine P. | |
dc.date.accessioned | 2020-10-20T19:10:38Z | |
dc.date.available | 2020-10-20T19:10:38Z | |
dc.date.issued | 2020-09-24 | |
dc.identifier | 10.3389/fcimb.2020.00482 | |
dc.identifier.issn | 22352988 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12728/7063 | |
dc.description.abstract | Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly described in Vibrio cholerae, is a secreted toxin that increases intestinal permeability. Recently, we identified Zot-encoding genes in the genomes of highly cytotoxic Chilean V. parahaemolyticus strains, including the non-toxigenic clinical strain PMC53.7. To gain insights into a possible role of Zot in V. parahaemolyticus, we analyzed whether it could be responsible for cytotoxicity. However, we observed a barely positive correlation between Caco-2 cell membrane damage and Zot mRNA expression during PMC53.7 infection and non-cytotoxicity induction in response to purified PMC53.7-Zot. Unusually, we observed a particular actin disturbance on cells infected with PMC53.7. Based on this observation, we decided to compare the sequence of PMC53.7-Zot with Zot of human pathogenic species such as V. cholerae, Campylobacter concisus, Neisseria meningitidis, and other V. parahaemolyticus strains, using computational tools. The PMC53.7-Zot was compared with other toxins and identified as an endotoxin with conserved motifs in the N-terminus and a variable C-terminal region and without FCIGRL peptide. Notably, the C-terminal diversity among Zots meant that not all of them could be identified as toxins. Structurally, PMC53.7-Zot was modeled as a transmembrane protein. Our results suggested that it has partial 3D structure similarity with V. cholerae-Zot. Probably, the PMC53.7-Zot would affect the actin cytoskeletal, but, in the absence of FCIGRL, the mechanisms of actions must be elucidated. | es_ES |
dc.language.iso | en | es_ES |
dc.publisher | Frontiers Media S.A. | es_ES |
dc.subject | Campylobacter concisus | es_ES |
dc.subject | intestinal permeability | es_ES |
dc.subject | non-toxigenic strains | es_ES |
dc.subject | Protein structure prediction | es_ES |
dc.subject | Vibrio cholerae | es_ES |
dc.subject | Vibrio parahaemolyticus | es_ES |
dc.subject | Zonula occludens toxin | es_ES |
dc.subject | Zot | es_ES |
dc.title | Analysis of the Zonula occludens Toxin Found in the Genome of the Chilean Non-toxigenic Vibrio parahaemolyticus Strain PMC53.7 | es_ES |
dc.type | Article | es_ES |