Mostrar el registro sencillo del ítem

Characterization of a Novel Drosophila SERT Mutant: Insights on the Contribution of the Serotonin Neural System to Behaviors

dc.contributor.authorHidalgo S.
dc.contributor.authorMolina-Mateo D.
dc.contributor.authorEscobedo P.
dc.contributor.authorZárate R.V.
dc.contributor.authorFritz E.
dc.contributor.authorFierro A.
dc.contributor.authorPerez E.G.
dc.contributor.authorIturriaga-Vasquez P.
dc.contributor.authorReyes-Parada M.
dc.contributor.authorVaras R.
dc.contributor.authorFuenzalida-Uribe N.
dc.contributor.authorCampusano J.M.
dc.date.accessioned2020-09-02T22:20:30Z
dc.date.available2020-09-02T22:20:30Z
dc.date.issued2017
dc.identifier10.1021/acschemneuro.7b00089
dc.identifier.citation8, 10, 2168-2179
dc.identifier.issn19487193
dc.identifier.urihttps://hdl.handle.net/20.500.12728/4896
dc.descriptionA better comprehension on how different molecular components of the serotonergic system contribute to the adequate regulation of behaviors in animals is essential in the interpretation on how they are involved in neuropsychiatric and pathological disorders. It is possible to study these components in "simpler" animal models including the fly Drosophila melanogaster, given that most of the components of the serotonergic system are conserved between vertebrates and invertebrates. Here we decided to advance our understanding on how the serotonin plasma membrane transporter (SERT) contributes to serotonergic neurotransmission and behaviors in Drosophila. In doing this, we characterized for the first time a mutant for Drosophila SERT (dSERT) and additionally used a highly selective serotonin-releasing drug, 4-methylthioamphetamine (4-MTA), whose mechanism of action involves the SERT protein. Our results show that dSERT mutant animals exhibit an increased survival rate in stress conditions, increased basal motor behavior, and decreased levels in an anxiety-related parameter, centrophobism. We also show that 4-MTA increases the negative chemotaxis toward a strong aversive odorant, benzaldehyde. Our neurochemical data suggest that this effect is mediated by dSERT and depends on the 4-MTA-increased release of serotonin in the fly brain. Our in silico data support the idea that these effects are explained by specific interactions between 4-MTA and dSERT. In sum, our neurochemical, in silico, and behavioral analyses demonstrate the critical importance of the serotonergic system and particularly dSERT functioning in modulating several behaviors in Drosophila. © 2017 American Chemical Society.
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.subjectamine release
dc.subjectcentrophobism
dc.subjectDrosophila
dc.subjectmotor behavior
dc.subjectolfaction
dc.subjectSERT
dc.subject4 methylthioamphetamine
dc.subjectamphetamine derivative
dc.subjectserotonin
dc.subjectserotonin transporter
dc.subjectunclassified drug
dc.subjectDrosophila protein
dc.subjectserotonin
dc.subjectserotonin receptor affecting agent
dc.subjectserotonin transporter
dc.subjectSerT protein, Drosophila
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectbrain tissue
dc.subjectchemotaxis
dc.subjectcontrolled study
dc.subjectDrosophila
dc.subjectlocomotion
dc.subjectmale
dc.subjectneurotransmission
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectserotonin release
dc.subjectserotoninergic system
dc.subjectsurvival rate
dc.subjectanimal
dc.subjectanimal behavior
dc.subjectbrain
dc.subjectDrosophila melanogaster
dc.subjectdrug effect
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectmutation
dc.subjectAnimals
dc.subjectBehavior, Animal
dc.subjectBrain
dc.subjectDrosophila melanogaster
dc.subjectDrosophila Proteins
dc.subjectMutation
dc.subjectSerotonin
dc.subjectSerotonin Agents
dc.subjectSerotonin Plasma Membrane Transport Proteins
dc.titleCharacterization of a Novel Drosophila SERT Mutant: Insights on the Contribution of the Serotonin Neural System to Behaviors
dc.typeArticle


Ficheros en el ítem

Thumbnail
Nombre:
item_2-s2.0-85031690750.pdf
Tamaño:
4.055Kb
Formato:
PDF
Ver/

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem