Valp1, a Newly Identified Temperate Phage Facilitating Coexistence of Lysogenic and Non-Lysogenic Populations of Vibrio anguillarum
Autor
Arce, Manuel
Venegas, Guillermo
Paez, Karla
Latz, Simone
Navarrete, Paola
Caruffo, Mario
Feijoo, Carmen
García, Katherine
Bastías, Roberto
Resumen
Vibrio anguillarum is a pathogen for several fish and shellfish species. Its ecology is influenced by diverse factors, including bacteriophages. Here, we identify and characterize a new temperate bacteriophage (Valp1) of V. anguillarum. Valp1 is a myovirus with a 60 nm head and a 90 nm contractile tail. Its double-stranded DNA genome of 42,988 bp contains 68 genes, including a protelomerase gene, typical of telomeric phages. Valp1 inhibits the growth of the virulent strain of V. anguillarum PF4, while the derived lysogenic strain P1.1 presents a slight reduction in its growth but is not affected by the presence of Valp1. Both strains present similar virulence in a larval zebrafish (Danio rerio) model, and only slight differences have been observed in their biochemical profile. Co-culture assays reveal that PF4 and P1.1 can coexist for 10 h in the presence of naturally induced Valp1, with the proportion of PF4 ranging between 28% and 1.6%. By the end of the assay, the phage reached a concentration of ~108 PFU/mL, and all the non-lysogenic PF4 strains were resistant to Valp1. This equilibrium was maintained even after five successive subcultures, suggesting the existence of a coexistence mechanism between the lysogenic and non-lysogenic populations of V. anguillarum in conjunction with the phage Valp1. © 2024 by the authors.
Colecciones
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Article
Probiotic Yeasts and Vibrio anguillarum Infection Modify the Microbiome of Zebrafish Larvae (2021)
Vargas, Orlando; Gutiérrez, María Soledad; Caruffo, Mario; Valderrama, Benjamín; Medina, Daniel A.; García, Katherine P.; Reyes-Jara, Angélica; Toro, Magaly A.; Feijoo, Carmen G.; Navarrete, Paola (Frontiers Media S.A., 2021-06-23)The host microbiome plays an essential role in health and disease. Microbiome modification by pathogens or probiotics has been poorly explored especially in the case of probiotic yeasts. Next-generation sequencing currently ... -
Article
Analysis of the Zonula occludens Toxin Found in the Genome of the Chilean Non-toxigenic Vibrio parahaemolyticus Strain PMC53.7 (2020)
Pérez-Reytor, Diliana Celeste; Pavón, Alequis; López-Joven, Carmen; Ramírez-Araya, Sebastián; Peña-Varas, Carlos; Plaza, Nicolás; Alegría-Arcos, Melissa; Corsini, Gino; Jaña, Víctor; Pavez, Leonardo; ... (Frontiers Media S.A., 2020-09-24)Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly ... -
Article
The High Risk of Bivalve Farming in Coastal Areas With Heavy Metal Pollution and Antibiotic-Resistant Bacteria: A Chilean Perspective (2024)
Pavón, Alequis; Riquelme, Diego; Jaña, Víctor; Iribarren, Cristian; Manzano, Camila; Lopez-Joven, Carmen; Reyes-Cerpa, Sebastián; Navarrete, Paola; Pavez, Leonardo; García, Katherine (Frontiers Media S.A., 2022)Anthropogenic pollution has a huge impact on the water quality of marine ecosystems. Heavy metals and antibiotics are anthropogenic stressors that have a major effect on the health of the marine organisms. Although heavy ...