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Development of a Multiplex PCR Assay for the Detection of Metallo-Beta-Lactamase Genes in Pseudomonas aeruginosa
dc.contributor.author | Luengo, Alexis | |
dc.contributor.author | Navarro-Heredia, Laura | |
dc.contributor.author | Rabert, Claudia | |
dc.contributor.author | Cortés-Cortés, Piedad | |
dc.contributor.author | Karahanian, Eduardo | |
dc.contributor.author | Corsini, Gino | |
dc.date.accessioned | 2024-04-10T01:54:32Z | |
dc.date.available | 2024-04-10T01:54:32Z | |
dc.date.issued | 2023 | |
dc.identifier | 10.4067/S0717-95022023000200466 | |
dc.identifier.issn | 07179367 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12728/10714 | |
dc.description.abstract | The appearance of Pseudomonas aeruginosa strains with multi-resistance to antibiotics is a clinical problem of great relevance. The methods for detecting these resistances are laborious and slow, which is a complication when treating patients promptly. In this work, we developed a simple method for simultaneous detection of several carbapenem resistance genes using a multiplex PCR assay. The PCR assay developed, followed by electrophoretic separation of fragments, allows to simultaneously identify the presence of 6 antibiotic resistance genes: bla-VIM (261 bp), bla-IMP (587 bp), bla-SPM (648 bp), bla-GIM-1 (753 bp), bla-NDM-1 (813 bp) and bla-KPC (882 bp). We analyzed 7 clinical isolates of P. aeruginosa obtained in Chile, finding the resistance genes bla-VIM, bla-IMP, bla-SPM, bla-GIM, and bla-NDM in 5 of them. We found a perfect correlation between the detection of various resistance genes by PCR and the results obtained by antibiograms. Interestingly, 2 of the strains possessed 3 different resistance genes simultaneously. Finally, in this work, we found the presence of 3 genes never described before in clinical isolates of P. aeruginosa in Chile (bla-IMP, bla-SPM, and bla-GIM-1). We developed a rapid multiplex PCR test for the simultaneous detection of up to 6 antibiotic resistance genes of the metallo--lactamase family in P. aeruginosa. © 2023, Universidad de la Frontera. All rights reserved. | es_ES |
dc.description.sponsorship | Universidad Autónoma de Coahuila, UAdeC, (DIUA189-2020); Fondo Nacional de Desarrollo Científico y Tecnológico, FONDECYT, (11090182, 11181270); Agencia Nacional de Investigación y Desarrollo, ANID | es_ES |
dc.language.iso | en | es_ES |
dc.publisher | Universidad de la Frontera | es_ES |
dc.subject | Antibiotic resistance | es_ES |
dc.subject | Carbapenem | es_ES |
dc.subject | Multiplex PCR | es_ES |
dc.subject | P. aeruginosa | es_ES |
dc.title | Development of a Multiplex PCR Assay for the Detection of Metallo-Beta-Lactamase Genes in Pseudomonas aeruginosa | es_ES |
dc.title.alternative | Desarrollo de un PCR Múltiple para la Detección de Genes Metalo-Beta-Lactamasa en Pseudomonas aeruginosa | es_ES |
dc.type | Article | es_ES |