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dc.contributor.authorRubio, Julia
dc.contributor.authorArias, Guisselle
dc.contributor.authorRobles-Kelly, Christian
dc.contributor.authorSilva-Moreno, Evelyn Del C.
dc.contributor.authorEspinoza, Luis J.
dc.contributor.authorCarrasco, Héctor
dc.contributor.authorOlea, Andres F.
dc.date.accessioned2022-03-14T17:04:33Z
dc.date.available2022-03-14T17:04:33Z
dc.date.issued2022-02-02
dc.identifier10.3390/plants11040546
dc.identifier.issn22237747
dc.identifier.urihttps://hdl.handle.net/20.500.12728/9971
dc.description.abstractPlant cell culture is a source of plant material from which bioactive metabolites can be extracted. In this work, the in vitro propagation of Leptocarpha rivularis, an endemic Chilean shrub with anticancer activity, is described. Different media were tested and optimized for the introduction, propagation, and rooting steps of the micropropagation process. At the end of this process, 83% of plants were successfully acclimatized under greenhouse conditions. Callus induction from the internodal stem segment was performed using various combinations of phytohormones. Green-colored, friable, and non-organogenic callus was generated with a callus induction index higher than 90%. The chemical composition of extracts and callus, obtained from clonal plants, was assessed and the results indicate that the phytochemical profiles of extracts from micropropagated plants are like those found for plants collected from natural habitats, leptocarpine (LTC) being the major component. However, no LTC was detected in callus extract. HeLa and CoN cells, treated with LTC or extract of micropropagated plants, exhibit important diminution on cell viability and a drastic decrease in gene expression of IL-6 and mmp2, genes associated with carcinogenic activity. These effects are more important in cancer cells than in normal cells. Thus, micropropagated L. rivularis could be developed as a potential source of efficient antiproliferative agents.es_ES
dc.language.isoenes_ES
dc.publisherMDPIes_ES
dc.subjectCallogenesises_ES
dc.subjectCell viabilityes_ES
dc.subjectGene expressiones_ES
dc.subjectMicropropagationes_ES
dc.subjectPhytochemistryes_ES
dc.titlePhytochemical Profiling and Assessment of Anticancer Activity of Leptocarpha rivularis Extracts Obtained from In Vitro Cultureses_ES
dc.typeArticlees_ES


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