New nanocomposite made of cashew apple bagasse lignin and Fe3O4 for immobilizing of lipase B from Candida antarctica aiming at esterification
MetadataShow full item record
Serpa, Juliana De França
Matias, Gabriel A.B.
Almeida Fechine, Pierre Basílio
da Costa, Victor M.
Melo Freire, Rafael
Casagrande Denardin, Juliano
Barros Gonçalves, Luciana Rocha
Casimiro De Macedo, André
Valderez Ponte Rocha, Maria
BACKGROUND: Novel magnetite-lignin hybrid materials were synthesized using lignin from cashew apple bagasse (CAB) and iron oxide nanoparticles (magnetite, Fe3O4). The new material, termed MNs/Lig (magnetite conjugated with lignin), was used as a support for the immobilization of lipase B from Candida antarctica (CAL─B), aiming to obtain an active and stable biocatalyst that is easily recoverable from the reactional medium. RESULTS: The magnetite/lignin composite showed a higher thermal stability than pure lignin, and it presented good magnetic properties, both before and after the immobilization procedure. The immobilization yield was higher using the unaltered MN/Lig as a support than when using the glutaraldehyde-activated MNs/Lig (MNs/Lig_GA), with yields of 66.6% and 40.0%, respectively. The presence of Triton X-100 did not influence the immobilization yield or the thermal stability of the biocatalyst when employing the unaltered MNs/Lig. However, it influenced the thermal stability of the lipase immobilized on MNs/Lig_GA, lowering the biocatalyst stability, which showed a half-life 11-fold lower than that of MNs/Lig_GA_CALB. The biocatalysts were tested with regards to the synthesis of ethyl oleate using oleic acid and ethanol as substrates, and 2-ethylhexyl oleate (a biolubricant) using oleic acid and 2-ethyl-hexanol as substrate. The conversions obtained by MNs/Lig_Tri_CALB were 88.2% and 76.7% for ethyl oleate and 2-ethylhexyl oleate, respectively. CONCLUSION: The novel strategy presented in this work for obtaining a biocatalyst using synthesized hybrids (MNs/Lig) may be a promising route for lipase immobilization because it can be considered environmentally benign and it shows a strong potential use in several reactions of industrial interest.
Showing items related by title, author, creator and subject.
Hydroxyapatite-based magnetic bionanocomposite as pharmaceuticals carriers in chitosan scaffolds (2021) Chaves, Anderson Valério; Melo Freire, Rafael; Feitosa, V. P.; Silva Ricardo, Nagila Maria Pontes; Casagrande Denardin, Juliano; Andrade Neto, Davino Machado; Almeida Fechine, Pierre Basílio (MDPI AG, 2021)Hydroxyapatite (HA) is a bioceramic very similar to the mineral component of bones and teeth. It is well established that osteoblasts grow better onto HA-coated metals than on metals alone. Herein, the preparation of a new ...
Articlede Menezes, Fernando Lima; Andrade Neto, Davino Machado; Linhares Rodrigues, Maria Do Livramento; Silva Lima, Helder Levi; Paiva, D. V.M.; Santos da Silva, Marcelo Antônio; Uchoa Dutra Fechine, Lillian Maria; Bezerra Sombra, Antônio Sérgio; Melo Freire, Rafael; Casagrande Denardin, Juliano; ... (MDPI AG, 2020)Magneto-dielectric composites are interesting advanced materials principally due to their potential applications in electronic fields, such as in microstrip antennas substrates. In this work, we developed superparamagnetic ...
Organophosphorus halloysite nanotubes as adsorbent for lead preconcentration in wine and grape juice (2020) Santana, Allan M.; Melo Freire, Tiago; da Silva, Francisco L.F.; Menezes, Fernando L.; Ramos, Lauren L.; Cavalcante, Giovanna R.; Dutra Fechine, Lillian Maria Uchoa; Sousa Vieira, Laís Helena; Ferreira, Odair P.; Melo Freire, Rafael; ... (Elsevier Ltd, 2020-11-04)Preconcentration of Pb(II) in grape-based beverages using organophosphate-functionalized halloysite nanotubes was investigated by flame atomic absorption spectroscopy (FAAS). For this proposal, a central composition design ...