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dc.contributor.authorSalazar C.
dc.contributor.authorElorza A.A.
dc.contributor.authorCofre G.
dc.contributor.authorRuiz-Hincapie P.
dc.contributor.authorShirihai O.
dc.contributor.authorRuiz L.M.
dc.date.accessioned2020-09-02T22:27:39Z
dc.date.available2020-09-02T22:27:39Z
dc.date.issued2019
dc.identifier10.1002/jcp.28362
dc.identifier.citation234, 10, 17405-17419
dc.identifier.issn00219541
dc.identifier.urihttps://hdl.handle.net/20.500.12728/6151
dc.descriptionHIG2A promotes cell survival under hypoxia and mediates the assembly of complex III and complex IV into respiratory chain supercomplexes. In the present study, we show that human HIGD2A and mouse Higd2a gene expressions are regulated by hypoxia, glucose, and the cell cycle-related transcription factor E2F1. The latter was found to bind the promoter region of HIGD2A. Differential expression of the HIGD2A gene was found in C57BL/6 mice in relation to tissue and age. Besides, the silencing of HIGD2A evidenced the modulation of mitochondrial dynamics proteins namely, OPA1 as a fusion protein increases, while FIS1, a fission protein, decreases. Besides, the mitochondrial membrane potential (ΔΨm) increased. The protein HIG2A is localized in the mitochondria and nucleus. Moreover, we observed that the HIG2A protein interacts with OPA1. Changes in oxygen concentration, glucose availability, and cell cycle regulate HIGD2A expression. Alterations in HIGD2A expression are associated with changes in mitochondrial physiology. © 2019 Wiley Periodicals, Inc.
dc.language.isoen
dc.publisherWiley-Liss Inc.
dc.subjectcell cycle
dc.subjectE2F1
dc.subjectHIG2A
dc.subjecthypoxia
dc.subjectmitochondrial dynamics
dc.subjectOPA1
dc.subjectOXPHOS supercomplexes
dc.subjectcytochrome c oxidase
dc.subjectglucose
dc.subjectRNA directed DNA polymerase
dc.subjecttranscription factor E2F1
dc.subjectubiquinol cytochrome c reductase
dc.subjectHIG2 protein, mouse
dc.subjectHILPDA protein, human
dc.subjectmitochondrial protein
dc.subjectreduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone)
dc.subjecttumor protein
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectcell cycle
dc.subjectcell hypoxia
dc.subjectcell survival
dc.subjectcontrolled study
dc.subjectenergy metabolism
dc.subjectgene
dc.subjectgene expression
dc.subjectHEK293 cell line
dc.subjecthigd2a gene
dc.subjectimmunofluorescence
dc.subjectimmunoprecipitation
dc.subjectmale
dc.subjectmitochondrial dynamics
dc.subjectmitochondrial membrane potential
dc.subjectmitochondrion
dc.subjectmouse
dc.subjectneuroblastoma cell
dc.subjectnonhuman
dc.subjectoxidative phosphorylation
dc.subjectoxidative phosphorylation system
dc.subjectoxygen concentration
dc.subjectpriority journal
dc.subjectpromoter region
dc.subjectreal time polymerase chain reaction
dc.subjectrespiratory chain
dc.subjectWestern blotting
dc.subjectanimal
dc.subjectC57BL mouse
dc.subjectcell cycle
dc.subjectgenetics
dc.subjecthuman
dc.subjectmetabolism
dc.subjectmitochondrial membrane
dc.subjectphysiology
dc.subjectAnimals
dc.subjectCell Cycle
dc.subjectElectron Transport Complex I
dc.subjectHumans
dc.subjectMembrane Potential, Mitochondrial
dc.subjectMice, Inbred C57BL
dc.subjectMitochondrial Dynamics
dc.subjectMitochondrial Membranes
dc.subjectMitochondrial Proteins
dc.subjectNeoplasm Proteins
dc.titleThe OXPHOS supercomplex assembly factor HIG2A responds to changes in energetic metabolism and cell cycle
dc.typeArticle


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