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dc.contributor.authorRojas L.
dc.contributor.authorMelgar-Rodríguez S.
dc.contributor.authorDíaz-Zúñiga J.
dc.contributor.authorAlvarez C.
dc.contributor.authorMonasterio G.
dc.contributor.authorRojas C.
dc.contributor.authorCafferata E.A.
dc.contributor.authorHernández M.
dc.contributor.authorCortéz C.
dc.contributor.authorCarvajal P.
dc.contributor.authorVernal R.
dc.date.accessioned2020-09-02T22:27:09Z
dc.date.available2020-09-02T22:27:09Z
dc.date.issued2020
dc.identifier10.1111/odi.13237
dc.identifier.citation26, 2, 409-418
dc.identifier.issn1354523X
dc.identifier.urihttps://hdl.handle.net/20.500.12728/6068
dc.descriptionObjective: The serotype b of Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) induces higher cytokine production in dendritic cells (DCs) compared with the other serotypes. However, this increased immunostimulatory potential was modified when DCs were co-infected with the other A. actinomycetemcomitans serotypes. This study aimed to analyze whether the production of interferon gamma (IFN-γ), C-reactive protein (CRP), matrix metalloproteinase (MMP)-2, and MMP-9, as well as the activity of osteoclasts, also varies when DCs are co-infected with the A. actinomycetemcomitans serotypes. Materials and Methods: Human DCs were stimulated with the A. actinomycetemcomitans serotypes using the following stimulatory conditions: serotype a/b/c/a+b/a+c/b+c/a+b+c. The IFN-γ, CRP, and MMP-2 levels were quantified by ELISA. The active form of MMP-9 was quantified using fluorescent functional assays. The MMP-2 gelatinolytic activity was identified by zymogram. The osteoclast activity was determined by quantifying the TRAP expression and resorption-pit formation using cytochemistry and osteoassays. Results: Higher levels of IFN-γ, CRP, MMP-2, MMP-9, and osteoclast activity were detected when DCs were stimulated with the serotype b of A. actinomycetemcomitans compared with the others. This increased immunostimulatory potential attributed to serotype b diminished when DCs were co-infected with the serotype a. Conclusions: This study provides new insights into the virulence of A. actinomycetemcomitans and reveals important differences in the immunostimulatory and pro-destructive potential among its serotypes. © 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved
dc.language.isoen
dc.publisherBlackwell Publishing Ltd
dc.subjectA. actinomycetemcomitans
dc.subjectbone resorption
dc.subjectcytokines
dc.subjectmatrix metalloproteinases
dc.subjectosteoclasts
dc.subjectserotypes
dc.subjectC reactive protein
dc.subjectgamma interferon
dc.subjectgelatinase A
dc.subjectgelatinase B
dc.subjectC reactive protein
dc.subjectgamma interferon
dc.subjectgelatinase A
dc.subjectgelatinase B
dc.subjectMMP2 protein, human
dc.subjectMMP9 protein, human
dc.subjectAggregatibacter actinomycetemcomitans
dc.subjectArticle
dc.subjectbacterial virulence
dc.subjectcontrolled study
dc.subjectcytochemistry
dc.subjectdendritic cell
dc.subjectenzyme linked immunosorbent assay
dc.subjecthuman
dc.subjecthuman cell
dc.subjectnonhuman
dc.subjectosteoclast activity
dc.subjectosteolysis
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectserotype
dc.subjectzymography
dc.subjectAggregatibacter actinomycetemcomitans
dc.subjectclassification
dc.subjectmetabolism
dc.subjectmicrobiology
dc.subjectosteoclast
dc.subjectpathogenicity
dc.subjectserotype
dc.subjectAggregatibacter actinomycetemcomitans
dc.subjectC-Reactive Protein
dc.subjectDendritic Cells
dc.subjectHumans
dc.subjectInterferon-gamma
dc.subjectMatrix Metalloproteinase 2
dc.subjectMatrix Metalloproteinase 9
dc.subjectOsteoclasts
dc.subjectSerogroup
dc.titleInhibitory effect of serotype a of Aggregatibacter actinomycetemcomitans on the increased destructive potential of serotype b
dc.typeArticle


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