Universidad Autónoma de Chile
  • About
  • How to publish
  • Contact Us
    • Login

    Repositorio Académico

    Advanced search
    View Item 
    •   Home
    • Investigación y Desarrollo
    • Producción Científica
    • Artículos científicos
    • View Item
    •   Home
    • Investigación y Desarrollo
    • Producción Científica
    • Artículos científicos
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Morphological Changes in a Severe Model of Parkinson’s Disease and Its Suitability to Test the Therapeutic Effects of Microencapsulated Neurotrophic Factors

    Article
    View/Open
    item_2-s2.0-84995388316.pdf (4.492Kb)
    Date
    2017
    DOI
    10.1007/s12035-016-0244-1

    Metadata
    Show full item record
    Author
    Requejo C.
    Ruiz-Ortega J.A.
    Bengoetxea H.
    García-Blanco A.
    Herrán E.
    Aristieta A.
    Igartua M.
    Pedraz J.L.
    Ugedo L.
    Hernández R.M.
    Lafuente J.V.
    Abstract
    The unilateral 6-hydroxydopamine (6-OHDA) lesion of medial forebrain bundle (MFB) in rats affords us to study the advanced stages of Parkinson’s disease (PD). Numerous evidences suggest synergic effects when various neurotrophic factors are administered in experimental models of PD. The aim of the present work was to assess the morphological changes along the rostro-caudal axis of caudo-putamen complex and substantia nigra (SN) in the referred model in order to test the suitability of a severe model to evaluate new neurorestorative therapies. Administration of 6-OHDA into MFB in addition to a remarkable depletion of dopamine in the nigrostriatal system induced an increase of glial fibrillary acidic protein (GFAP)-positive cells in SN and an intense immunoreactivity for OX-42, vascular endothelial growth factor (VEGF), and Lycopersycum esculentum agglutinin (LEA) in striatum and SN. Tyrosine hydroxylase (TH) immunostaining revealed a significant decrease of the TH-immunopositive striatal volume in 6-OHDA group from rostral to caudal one. The loss of TH-immunoreactive (TH-ir) neurons and axodendritic network (ADN) was higher in caudal sections. Morphological recovery after the implantation of microspheres loaded with VEGF and glial cell line-derived neurotrophic factor (GDNF) in parkinsonized rats was related to the preservation of the TH-ir cell number and ADN in the caudal region of the SN. In addition, these findings support the neurorestorative role of VEGF+GDNF in the dopaminergic system and the synergistic effect between both factors. On the other hand, a topological distribution of the dopaminergic system was noticeable in the severe model, showing a selective vulnerability to 6-OHDA and recovering after treatment. © 2016, Springer Science+Business Media New York.
    Collections
    • Artículos científicos

    Related items

    Showing items related by title, author, creator and subject.

    • Article

      Deleterious effects of VEGFR2 and RET inhibition in a preclinical model of Parkinson’s disease (2020) 

      Requejo C.; Ruiz-Ortega J.A.; Bengoetxea H.; Bulnes S.; Ugedo L.; Lafuente J.V. (Humana Press Inc., 2018)
    • Article

      Nanodelivery of cerebrolysin and rearing in enriched environment induce neuroprotective effects in a preclinical rat model of Parkinson’s disease (2020) 

      Requejo C.; Ruiz-Ortega J.A.; Cepeda H.; Sharma A.; Sharma H.S.; Ozkizilcik A.; Tian R.; Moessler H.; Ugedo L.; Lafuente J.V. (Humana Press Inc., 2018)
    • Article

      Role of microfluidics in blood-brain barrier permeability cell culture modeling: Relevance to CNS disorders (2020) 

      Rusanov A.L.; Luzgina N.G.; Barreto G.E.; Aliev G. (Bentham Science Publishers, 2016)
    LA UNIVERSIDAD
    • Aseguramiento de Calidad
    • Internacional
    • Vinculación con el Medio
    • Más Transparencia
    • Acreditación
    • Reglamentos e instructivos
    • Reclamos y Sugerencias
    FACULTADES
    • Administración y Negocios
    • Arquitectura y Construcción
    • Ciencias de la Salud
    • Ciencias Sociales y Humanidades
    • Derecho
    • Educación
    • Ingeniería
    INVESTIGACIÓN
    • Estructura
    • Comités de Ética
    • Institutos de Investigación
    • Centros de Investigación
    • Grupos de Investigación
    • Docentes Investigadores
    POSTGRADOS
    • Postgrados
    • Doctorados
    • Magíster
    • Especialidades
    • Escuela Internacional
    • Escuela Alta Dirección

    Browse

    All contentCollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister
    Academic Journals
    LA UNIVERSIDAD
    • Aseguramiento de Calidad
    • Internacional
    • Vinculación con el Medio
    • Más Transparencia
    • Acreditación
    • Reglamentos e instructivos
    • Reclamos y Sugerencias
    FACULTADES
    • Administración y Negocios
    • Arquitectura y Construcción
    • Ciencias de la Salud
    • Ciencias Sociales y Humanidades
    • Derecho
    • Educación
    • Ingeniería
    INVESTIGACIÓN
    • Estructura
    • Comités de Ética
    • Institutos de Investigación
    • Centros de Investigación
    • Grupos de Investigación
    • Docentes Investigadores
    POSTGRADOS
    • Postgrados
    • Doctorados
    • Magíster
    • Especialidades
    • Escuela Internacional
    • Escuela Alta Dirección