Indirect immunofluorescence technique versus polymerase chain reaction for the diagnosis of respiratory viruses in children admitted to a hospital in the Metropolitan Region [Inmunofluorescencia indirecta versus reacción de polimerasa en cadena para el diagnóstico de virus respiratorios en niños ingresados en un hospital de la Región Metropolitana]
MetadataShow full item record
Pablo Corvalán L.
Guisselle Arias B.
Paola Morales S.
Raquel González M.
Jaime Inostroza S.
Loreto Fuenzalida I.
Background: Early viral detection in acute respiratory infections (ARI) is essential to establish appropriate therapy and prevent nosocomial transmission. Objective: To compare the efficacy of indirect immunofluorescence technique (IIF) with the polymerase chain reaction (PCR) to identify respiratory viruses in children hospitalized for ARI. Methods: 47 nasopharyngeal aspirates of children = 2 years with ARI were included. IFI included respiratory syncytial virus (RSV), adenovirus, influenza A and B and parainfluenza. PCR also included the detection of metapneumovirus, enterovirus/rhinovirus, bocavirus and coronavirus. Sensitivity, specificity, positive and negative predictive value (VPP/NPV) and kappa correlation for RSV were estimated by IIF compared to PCR. Results: The IIF detected only RSV (29; 61.7%). PCR detected several viruses, including RSV in 26 cases (55.3%), followed by bocavirus (29.8%), rhinovirus/enterovirus (21.3%), adenovirus (14.9%) and parainfluenza (4,3%) among others, with 35.5% of coinfection. The IIF presented sensitivity: 85.7%, specificity: 73.6%, PPV: 82.7%, NPV: 77.7% and kappa: 0.5990 (95% CI, 0.3636-0.8346) for RSV. Conclusion: The IIF presents good sensitivity, but moderate specificity for RSV. However, IIF fails to detect other respiratory viruses. The introduction of PCR would improve the etiological diagnosis of ARI of viral origin. © 2019, Sociedad Chilena de Infectologia. All rights reserved.
Showing items related by title, author, creator and subject.
Overnutrition in Infants Is Associated With High Level of Leptin, Viral Coinfection and Increased Severity of Respiratory Infections: A Cross-Sectional Study (2020) Arias-Bravo G.; Valderrama G.; Inostroza J.; Reyes-Farías M.; Garcia-Diaz D.F.; Zorondo-Rodríguez F.; Fuenzalida L.F. (Frontiers Media S.A., 2020)
Obesity as a risk factor for complications during acute respiratory infections in children [La relación entre obesidad y complicaciones en el curso clínico de las enfermedades respiratorias virales en niños ¿un nuevo factor de riesgo a considerar?] (2020) Fuenzalida L.; García-Díaz D.F. (Sociedad Medica de Santiago, 2016)
Psychometric properties of scales used for grading the severity of bronchial obstruction in pediatrics: A systematic review and meta-analysis (2020) Luarte-Martinez S.; Rodriguez-Núñez I.; Astudillo P.; Manterola C. (Sociedad Argentina de Pediatria, 2017)