Characterization of FCXTH2, a novel xyloglucan endotransglycosylase/hydrolase enzyme of chilean strawberry with hydrolase activity
MetadataShow full item record
Xyloglucan endotransglycosylase/hydrolases (XTHs) are cell wall enzymes with hydrolase (XEH) and/or endotransglycosylase (XET) activities. As they are involved in the modification of the xyloglucans, a type of hemicellulose present in the cell wall, they are believed to be very important in different processes, including growth, development, and fruit ripening. Previous studies suggest that XTHs might play a key role in development and ripening of Fragaria chiloensis fruit, and its characterization is pending. Therefore, in order to provide a biochemical characterization of the FcXTH2 enzyme to explain its possible role in strawberry development, the molecular cloning and the heterologous expression of FcXTH2 were performed. The recombinant FcXTH2 was active and displayed mainly XEH activity. The optimal pH and temperature are 5.5 and 37 °C, respectively. A KM value of 0.029 mg mL−1 was determined. Additionally, its protein structural model was built through comparative modeling methodology. The model showed a typically β-jelly-roll type folding in which the catalytic motif was oriented towards the FcXTH2 central cavity. Using molecular docking, protein-ligand interactions were explored, finding better interaction with xyloglucan than with cellulose. The data provided groundwork for understanding, at a molecular level, the enzymatic mechanism of FcXTH2, an important enzyme acting during the development of the Chilean strawberry. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Showing items related by title, author, creator and subject.
Structural analysis of the woodland strawberry COI1-JAZ1 co-receptor for the plant hormone jasmonoyl-isoleucine (2020) Valenzuela-Riffo F.; Garrido-Bigotes A.; Figueroa P.M.; Morales-Quintana L.; Figueroa C.R. (Elsevier Inc., 2018)
ReviewOjeda P.G.; Ramírez D.; Alzate-Morales J.; Caballero J.; Kaas Q.; González W. (MDPI AG, 2018)
Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit (2020) Valenzuela-Riffo F.; Gaete-Eastman C.; Stappung Y.; Lizana R.; Herrera R.; Moya-León M.A.; Morales-Quintana L. (Taylor and Francis Ltd., 2019)