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dc.contributor.authorMardones M.D.
dc.contributor.authorJorquera P.V.
dc.contributor.authorHerrera-Soto A.
dc.contributor.authorAmpuero E.
dc.contributor.authorBustos F.J.
dc.contributor.authorvan Zundert B.
dc.contributor.authorVarela-Nallar L.
dc.date.accessioned2020-09-02T22:22:21Z
dc.date.available2020-09-02T22:22:21Z
dc.date.issued2019
dc.identifier10.1016/j.jchemneu.2019.04.009
dc.identifier.citation98, , 117-123
dc.identifier.issn08910618
dc.identifier.urihttps://hdl.handle.net/20.500.12728/5205
dc.descriptionIn the adult hippocampus new neurons are generated in the dentate gyrus from neural progenitor cells. Adult-born neurons integrate into the hippocampal circuitry and contribute to hippocampal function. PSD95 is a major postsynaptic scaffold protein that is crucial for morphological maturation and synaptic development of hippocampal neurons. Here we study the function of PSD95 in adult hippocampal neurogenesis by downregulating PSD95 expression in newborn cells using retroviral-mediated RNA interference. Retroviruses coding for a control shRNA or an shRNA targeting PSD95 (shPSD95)were stereotaxically injected into the dorsal dentate gyrus of 2-month-old C57BL/6 mice. PSD95 knockdown did not affect neuronal differentiation of newborn cells into neurons, or migration of newborn neurons into the granule cell layer. Morphological analysis revealed that newborn neurons expressing shPSD95 showed increased dendritic length and increased number of high-order dendrites. Concomitantly, dendrites from shPSD95-expressing newborn granule neurons showed a reduction in the density of dendritic spines. These results suggest that PSD95 is required for proper dendritic and spine maturation of adult-born neurons, but not for early stages of neurogenesis in the hippocampus. © 2019 Elsevier B.V.
dc.language.isoen
dc.publisherElsevier B.V.
dc.subjectAdult
dc.subjectHippocampus
dc.subjectNeurogenesis
dc.subjectPSD95
dc.subjectdisks large homolog 4
dc.subjectshort hairpin RNA
dc.subjectdisks large homolog 4
dc.subjectDlg4 protein, mouse
dc.subjectadult
dc.subjectanimal cell
dc.subjectArticle
dc.subjectcell migration
dc.subjectcontrolled study
dc.subjectdendritic spine
dc.subjectdorsal dentate gyrus
dc.subjectdown regulation
dc.subjectfemale
dc.subjectgene knockdown
dc.subjectgene targeting
dc.subjectgranule cell
dc.subjecthippocampus
dc.subjectin vivo study
dc.subjectmale
dc.subjectmouse
dc.subjectnerve cell differentiation
dc.subjectnervous system development
dc.subjectnewborn
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectprotein function
dc.subjectRNA interference
dc.subjectadult stem cell
dc.subjectanimal
dc.subjectC57BL mouse
dc.subjectcell differentiation
dc.subjectcell motion
dc.subjectcytology
dc.subjecthippocampus
dc.subjectmetabolism
dc.subjectnerve cell
dc.subjectneural stem cell
dc.subjectphysiology
dc.subjectAdult Stem Cells
dc.subjectAnimals
dc.subjectCell Differentiation
dc.subjectCell Movement
dc.subjectDisks Large Homolog 4 Protein
dc.subjectHippocampus
dc.subjectMice
dc.subjectMice, Inbred C57BL
dc.subjectNeural Stem Cells
dc.subjectNeurogenesis
dc.subjectNeurons
dc.titlePSD95 regulates morphological development of adult-born granule neurons in the mouse hippocampus
dc.typeArticle


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