Universidad Autónoma de Chile
  • About
  • How to publish
  • Contact Us
    • Login

    Repositorio Académico

    Advanced search
    View Item 
    •   Home
    • Investigación y Desarrollo
    • Producción Científica
    • Artículos científicos
    • View Item
    •   Home
    • Investigación y Desarrollo
    • Producción Científica
    • Artículos científicos
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Arginase-endothelial nitric oxide synthase imbalance contributes to endothelial dysfunction during chronic intermittent hypoxia

    Article
    View/Open
    item_2-s2.0-84922343707.pdf (4.266Kb)
    Date
    2015
    DOI
    10.1097/HJH.0000000000000453

    Metadata
    Show full item record
    Author
    Krause B.J.
    Del Rio R.
    Moya E.A.
    Marquez-Gutierrez M.
    Casanello P.
    Iturriaga R.
    Abstract
    OBJECTIVE:: Chronic intermittent hypoxia (CIH), the main feature of obstructive sleep apnoea, is associated with impaired vascular function despite unaltered response to nitric oxide donors. This study addressed whether arginase contributes to the endothelial dysfunction in CIH rats. METHODS:: Adult male Sprague-Dawley rats were exposed for 21 days to CIH (5% oxygen, 12 times/h, 8h/day). The internal carotid arteries were isolated to study endothelial nitric oxide synthase (eNOS) and arginase-1 levels by western blot and immunohistochemistry, and their vasoactive responses using wire myography. Relaxation to sodium nitroprusside (SNP; nitric oxide donor) in the presence or absence of soluble guanylyl cyclase inhibitor, and acetylcholine with and without a NOS inhibitor [N-nitro-L-arginine (L-NA)] and the arginase inhibitor BEC were determined. RESULTS:: Arteries from the CIH rats presented higher active contraction induced by KCl (3.5±0.4 vs. 2.3±0.2N/m), augmented media-to-lumen ratio (∼40%), decreased relaxation to acetylcholine (12.8±1.5 vs. 30.5±4.6%) and increased sensitivity to SNP (pD2 7.3±0.1 vs. 6.7±0.1). Arginase inhibition reversed the impaired acetylcholine-induced relaxation in CIH arteries (49.5±7.4%), an effect completely blocked by L-NA. In the carotid arteries, arginase-1 protein level was increased, whereas eNOS levels decreased in the CIH arteries. CONCLUSION:: The current results suggest that endothelial dysfunction in CIH-induced hypertension may result from imbalanced arginase-1 to eNOS expression, vascular remodelling and increased contractile capacity, rather than decreased vascular response to nitric oxide. © 2015 Wolters Kluwer Health, Inc.
    Collections
    • Artículos científicos

    Related items

    Showing items related by title, author, creator and subject.

    • Article

      Carotid Body Ablation Abrogates Hypertension and Autonomic Alterations Induced by Intermittent Hypoxia in Rats (2020) 

      Del Rio R.; Andrade D.C.; Lucero C.; Arias P.; Iturriaga R. (Lippincott Williams and Wilkins, 2016)
    • Article

      Deleterious effects of VEGFR2 and RET inhibition in a preclinical model of Parkinson’s disease (2020) 

      Requejo C.; Ruiz-Ortega J.A.; Bengoetxea H.; Bulnes S.; Ugedo L.; Lafuente J.V. (Humana Press Inc., 2018)
    • Review

      Enhanced carotid body chemosensory activity and the cardiovascular alterations induced by intermittent hypoxia (2020) 

      Iturriaga R.; Andrade D.C.; Del Rio R. (Frontiers Media S.A., 2014)
    LA UNIVERSIDAD
    • Aseguramiento de Calidad
    • Internacional
    • Vinculación con el Medio
    • Más Transparencia
    • Acreditación
    • Reglamentos e instructivos
    • Reclamos y Sugerencias
    FACULTADES
    • Administración y Negocios
    • Arquitectura y Construcción
    • Ciencias de la Salud
    • Ciencias Sociales y Humanidades
    • Derecho
    • Educación
    • Ingeniería
    INVESTIGACIÓN
    • Estructura
    • Comités de Ética
    • Institutos de Investigación
    • Centros de Investigación
    • Grupos de Investigación
    • Docentes Investigadores
    POSTGRADOS
    • Postgrados
    • Doctorados
    • Magíster
    • Especialidades
    • Escuela Internacional
    • Escuela Alta Dirección

    Browse

    All contentCollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister
    Academic Journals
    LA UNIVERSIDAD
    • Aseguramiento de Calidad
    • Internacional
    • Vinculación con el Medio
    • Más Transparencia
    • Acreditación
    • Reglamentos e instructivos
    • Reclamos y Sugerencias
    FACULTADES
    • Administración y Negocios
    • Arquitectura y Construcción
    • Ciencias de la Salud
    • Ciencias Sociales y Humanidades
    • Derecho
    • Educación
    • Ingeniería
    INVESTIGACIÓN
    • Estructura
    • Comités de Ética
    • Institutos de Investigación
    • Centros de Investigación
    • Grupos de Investigación
    • Docentes Investigadores
    POSTGRADOS
    • Postgrados
    • Doctorados
    • Magíster
    • Especialidades
    • Escuela Internacional
    • Escuela Alta Dirección