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dc.contributor.authorGómez G.I.
dc.contributor.authorFalcon R.V.
dc.contributor.authorMaturana C.J.
dc.contributor.authorLabra V.C.
dc.contributor.authorSalgado N.
dc.contributor.authorRojas C.A.
dc.contributor.authorOyarzun J.E.
dc.contributor.authorCerpa W.
dc.contributor.authorQuintanilla R.A.
dc.contributor.authorOrellana J.A.
dc.date.accessioned2020-09-02T22:19:53Z
dc.date.available2020-09-02T22:19:53Z
dc.date.issued2018
dc.identifier10.3389/fncel.2018.00472
dc.identifier.citation12, , -
dc.identifier.issn16625102
dc.identifier.urihttps://hdl.handle.net/20.500.12728/4792
dc.descriptionA mounting body of evidence indicates that adolescents are specially more susceptible to alcohol influence than adults. However, the mechanisms underlying this phenomenon remain poorly understood. Astrocyte-mediated gliotransmission is crucial for hippocampal plasticity and recently, the opening of hemichannels and pannexons has been found to participate in both processes. Here, we evaluated whether adolescent rats exposed to ethanol exhibit changes in the activity of astrocyte hemichannels and pannexons in the hippocampus, as well as alterations in astrocyte arborization and cytokine levels. Adolescent rats were subjected to ethanol (3.0 g/kg) for two successive days at 48-h periods over 14 days. The opening of hemichannels and pannexons was examined in hippocampal slices by dye uptake, whereas hippocampal cytokine levels and astroglial arborization were determined by ELISA and Sholl analysis, respectively. We found that adolescent ethanol exposure increased the opening of connexin 43 (Cx43) hemichannels and pannexin-1 (Panx1) channels in astrocytes. Blockade of p38 mitogen-activated protein kinase (MAPK), inducible nitric oxide synthase (iNOS) and cyclooxygenases (COXs), as well as chelation of intracellular Ca 2+ , drastically reduced the ethanol-induced channel opening in astrocytes. Importantly, ethanol-induced Cx43 hemichannel and Panx1 channel activity was correlated with increased levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), IL-6 in the hippocampus, as well as with profound alterations in astrocyte arbor complexity. Thus, we propose that uncontrolled opening of astrocyte hemichannels and pannexons may contribute not only to the glial dysfunction and neurotoxicity caused by adolescent alcohol consumption, but also to the pathogenesis of alcohol use disorders in the adulthood. © 2018 Gómez, Falcon, Maturana, Labra, Salgado, Rojas, Oyarzun, Cerpa, Quintanilla and Orellana.
dc.language.isoen
dc.publisherFrontiers Media S.A.
dc.subjectAlcoholism
dc.subjectAstrocyte
dc.subjectConnexins
dc.subjectCytokines
dc.subjectGlia
dc.subjectHippocampus
dc.subjectPannexins
dc.subject4 (4 fluorophenyl) 2 (4 hydroxyphenyl) 5 (4 pyridyl)imidazole
dc.subjectconnexin 43
dc.subjectcyclooxygenase 1
dc.subjectcyclooxygenase 2
dc.subjectcytokine
dc.subjectgamma interferon
dc.subjectgap junction protein
dc.subjectglial fibrillary acidic protein
dc.subjectinducible nitric oxide synthase
dc.subjectinterleukin 1beta
dc.subjectinterleukin 6
dc.subjectmitogen activated protein kinase p38
dc.subjectpannexin 1
dc.subjectpannexon
dc.subjectprostaglandin E2
dc.subjectprostaglandin synthase
dc.subjecttumor necrosis factor
dc.subjectunclassified drug
dc.subjectadolescent
dc.subjectalcohol consumption
dc.subjectalcoholism
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectantibody detection
dc.subjectArticle
dc.subjectastrocyte
dc.subjectbrain dysfunction
dc.subjectcalcium cell level
dc.subjectcell structure
dc.subjectcell survival
dc.subjectconfocal microscopy
dc.subjectcontrolled study
dc.subjectenzyme linked immunosorbent assay
dc.subjectglia cell
dc.subjecthippocampus
dc.subjectimage analysis
dc.subjectimage processing
dc.subjectmale
dc.subjectmembrane channel
dc.subjectnerve cell plasticity
dc.subjectnerve fiber
dc.subjectnervous system inflammation
dc.subjectnonhuman
dc.subjectpathogenesis
dc.subjectrat
dc.subjectsignal transduction
dc.subjectsynaptic transmission
dc.subjecttoxicity and intoxication
dc.titleHeavy alcohol exposure activates astroglial hemichannels and pannexons in the hippocampus of adolescent rats: Effects on neuroinflammation and astrocyte arborization
dc.typeArticle


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