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dc.contributor.authorGarcía-Piqueras J.
dc.contributor.authorGarcía-Suárez O.
dc.contributor.authorGarcía-Mesa Y.
dc.contributor.authorGarcía-Fernandez B.
dc.contributor.authorQuirós L.M.
dc.contributor.authorCobo R.
dc.contributor.authorMartín-Biedma B.
dc.contributor.authorFeito J.
dc.contributor.authorVega J.A.
dc.date.accessioned2020-09-02T22:18:52Z
dc.date.available2020-09-02T22:18:52Z
dc.date.issued2020
dc.identifier10.1002/ar.24328
dc.identifier.citation303, 8, 2262-2273
dc.identifier.issn19328486
dc.identifier.urihttps://hdl.handle.net/20.500.12728/4645
dc.descriptionHeparan sulfate proteoglycans are pericellular/cell surface molecules involved in somatosensory axon guidance in the peripheral nervous system. However, the distribution of heparan sulfate proteoglycans in the extracellular matrix of human cutaneous sensory corpuscles is unknown. Immunohistochemistry and immunofluorescence assays were performed to define the localization of heparan sulfate proteoglycans in human cutaneous Meissner's and Pacinian corpuscles using two anti-heparan sulfate antibodies together with anti-S100 protein, anti-PGP9.5, anti-CD34 (to immunolabel basement membranes, Schwann cells, axon and the intermediate endoneurial layer of Pacinian corpuscles, respectively), anti-Type IV collagen, and anti-chondroitin sulfate antibodies. Heparan sulfate proteoglycans were colocalized with Type IV collagen in Meissner's corpuscles and were located in the outer core lamellae and capsule, but not in the inner core or the intermediate layer, in Pacinian corpuscles. Chondroitin sulfate was observed in the intermediate layer of Pacinian corpuscles but was never colocalized with heparan sulfate proteoglycans. The present results strongly suggest that heparan sulfate proteoglycans are associated with the basement membranes of the lamellar cells in Meissner's corpuscles and with the complex outer core capsule in Pacinian corpuscles. The functional significance of these results, if any, remains to be elucidated. © 2019 American Association for Anatomy
dc.language.isoen
dc.publisherBlackwell Publishing Inc.
dc.subjectbasement membrane
dc.subjectcutaneous sensory corpuscles
dc.subjectextracellular matrix
dc.subjectheparan sulfate proteoglycans
dc.subjecthuman
dc.subjectantibody
dc.subjectCD34 antibody
dc.subjectchondroitin sulfate
dc.subjectcollagen type 4
dc.subjectpgp9.5 anitbody
dc.subjectprotein S 100
dc.subjectproteoheparan sulfate
dc.subjectunclassified drug
dc.subjectadult
dc.subjectantibody labeling
dc.subjectArticle
dc.subjectaxon
dc.subjectbasement membrane
dc.subjecthuman
dc.subjectimmunofluorescence test
dc.subjectimmunohistochemistry
dc.subjectmeissner's corpuscles
dc.subjectPacini corpuscle
dc.subjectpriority journal
dc.subjectSchwann cell
dc.subjectskin
dc.titleHeparan sulfate in human cutaneous Meissner's and Pacinian corpuscles
dc.typeArticle


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