Repositorio Académico

The spleen is the largest peripheral lymphoid organ of the body so it is essential to know their morphological quantitative aspects in order to identify potential abnormalities. The aim of this study was to determine the parameters stereological spleen in normal two species commonly used in research such as the guinea pig and rat, to obtain quantitative patterns of normality, which will serve as a basis for future studies morphofunctional. 5 spleens were used for each species (Cavia porcellus and Rattus novergicus, Sprague Dawley) obtained from biotery the Universidad de La Frontera, Temuco, Chile. The spleens were dissected and fixed in formalin buffered to 10% and the volume is determined by the of Scherle method. 5 pieces were obtained through Orientator and these were included in paraplast. Each piece was performed histological cuts of 3 mm thick and separated 200 μ m each other, which were stained with H-E. The percentage of red pulp, white pulp and marginal zone in the Guinea pig was: 65.14%, 21.96% and 12.67% respectively, and in the rat 53.9% pulp red, 25.75% white pulp and 15.87% of marginal zone.The total number of follicles was 8.33 × 102 follicles and 5.73 × 102 follicles for the Guinea pig and rat, respectively. The results are consistent with those obtained by other authors might identify a pattern of quantitative percentage of splenic compartments of normality that could be considered for future studies morpho-functional.