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dc.contributor.authorDíaz-Zúñiga J.
dc.contributor.authorMelgar-Rodríguez S.
dc.contributor.authorMonasterio G.
dc.contributor.authorPujol M.
dc.contributor.authorRojas L.
dc.contributor.authorAlvarez C.
dc.contributor.authorCarvajal P.
dc.contributor.authorVernal R.
dc.date.accessioned2020-09-02T22:16:53Z
dc.date.available2020-09-02T22:16:53Z
dc.date.issued2017
dc.identifier10.1016/j.archoralbio.2017.02.008
dc.identifier.citation78, , 26-33
dc.identifier.issn00039969
dc.identifier.urihttps://hdl.handle.net/20.500.12728/4367
dc.descriptionObjective In Aggregatibacter actinomycetemcomitans, different serotypes have been described based on lipopolysaccharide (LPS) antigenicity. When T lymphocytes were stimulated with these serotypes, different patterns of T-helper (Th)1 and Th17-type of immune responses were reported. Recently, two new Th phenotypes have been described and named Th9 and Th22 lymphocytes; however, their role in the pathogenesis of periodontitis remains unclear. This study aimed to investigate the potential Th9 and/or Th22 lymphocyte responses when stimulated with autologous dendritic cells infected with different A. actinomycetemcomitans serotypes. Methods Monocyte-derived dendritic cells and naïve CD4+ T lymphocytes were obtained from healthy donors and stimulated with different serotypes of A. actinomycetemcomitans at a multiplicity of infection MOI = 102 or their purified LPS (10–50 ng/ml). The levels for the Th9 and Th22-associated cytokines, as well as the transcription factor master-switch genes implied in their differentiation Spi-B and AhR, were quantified by qPCR and ELISA. Results When stimulated with the serotype b of A. actinomycetemcomitans, higher levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α were detected in dendritic cells, as well as higher levels of IL-22 and AhR were detected in T lymphocytes, when compared with stimulation with the other serotypes. Conclusions The serotype b of A. actinomycetemcomitans has a higher capacity of trigger Th22-type of immune response in both dendritic cells and T lymphocytes. These data allow us to suggest that, when the serotype b of A. actinomycetemcomitans is a significant part of the subgingival biofilm, the Th22 polarization might be triggered within the periodontal lesion. © 2017 Elsevier Ltd
dc.language.isoen
dc.publisherElsevier Ltd
dc.subjectAggregatibacter actinomycetemcomitans
dc.subjectT-lymphocytes
dc.subjectTh22 cells
dc.subjectTh9 cells
dc.subjectcytokine
dc.subjectvirulence factor
dc.subjectAggregatibacter actinomycetemcomitans
dc.subjectbiofilm
dc.subjectcell differentiation
dc.subjecthelper cell
dc.subjecthuman
dc.subjectimmunology
dc.subjectpathogenicity
dc.subjectphenotype
dc.subjectsecretion (process)
dc.subjectserotype
dc.subjectTh17 cell
dc.subjectAggregatibacter actinomycetemcomitans
dc.subjectBiofilms
dc.subjectCell Differentiation
dc.subjectCytokines
dc.subjectHumans
dc.subjectPhenotype
dc.subjectSerogroup
dc.subjectT-Lymphocytes, Helper-Inducer
dc.subjectTh17 Cells
dc.subjectVirulence Factors
dc.titleDifferential human Th22-lymphocyte response triggered by Aggregatibacter actinomycetemcomitans serotypes
dc.typeArticle


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