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dc.contributor.authorSalazar, Lorena
dc.contributor.authorRivas, Elizabeth
dc.contributor.authorPavón, Alequis
dc.contributor.authorWiese, Guillermo
dc.contributor.authorManzano, Camila
dc.contributor.authorOrellana, Paz
dc.contributor.authorGutierrez-Moraga, Ana
dc.contributor.authorCortés-Cortés, Piedad
dc.contributor.authorCorsini, Gino
dc.date.accessioned2024-04-10T06:43:26Z
dc.date.available2024-04-10T06:43:26Z
dc.date.issued2022
dc.identifier10.4067/S0717-95022022000501276
dc.identifier.issn07179367
dc.identifier.urihttps://hdl.handle.net/20.500.12728/11069
dc.description.abstractBacteriocins are antimicrobial peptides of ribosomal synthesis secreted by bacteria. Among these, nisin stands out, which has potential uses in antibiotic therapies, as a food bio preservative and probiotics. Nisin has also been reported to have cytotoxicity on neoplastic cell lines, but there is little information on its effect on blood tumor cells. Due to the potential use that nisin presents, it is relevant to determine the toxicity it presents on tumor cell lines of the blood type. For this, hemolytic activity tests were carried out on human erythrocytes and toxicity on human peripheral blood mononuclear cells, determining that nisin does not have a toxic effect on this type of normal human blood cells. Cytotoxicity tests were also carried out with tumor cell lines (K562 and U937), to determine dose, exposure time and selectivity in the toxic effect of nisin on human tumor cells. These tests show that nisin shows cytotoxic activity on K562 and U937 cell lines at 72 h of exposure, at a concentration of 40 µg / mL, which corresponds to 100 times the minimum inhibitory concentration (MIC) used for its action on bacteria. When comparing the effect of nisin on human peripheral blood mononuclear cells with lymphoid and myeloid tumor lines (K562 and U937 respectively), a selective effect of nisin on blood tumor cells is observed. © 2022, Universidad de la Frontera. All rights reserved.es_ES
dc.description.sponsorshipUniversidad Autónoma de Coahuila, UAdeC; Fondo Nacional de Desarrollo Científico y Tecnológico, FONDECYT, (11090182); Agencia Nacional de Investigación y Desarrollo, ANIDes_ES
dc.language.isoeses_ES
dc.publisherUniversidad de la Fronteraes_ES
dc.subjectBacteriocines_ES
dc.subjectBlood tumor cellses_ES
dc.subjectCytotoxicityes_ES
dc.subjectK562 cell and U937 cellses_ES
dc.subjectNisines_ES
dc.titleIn vitro Cytotoxicity of the Antimicrobial Polypeptide Nisin on Blood Tumor Cellses_ES
dc.title.alternativeCitotoxicidad in vitro del Polipéptido Antimicrobiano Nisina sobre Células Tumorales Sanguíneases_ES
dc.typeArticlees_ES


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