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dc.contributor.authorGutiérrez, Mario Felipe
dc.contributor.authorAlegría-Acevedo, Luísa Fernanda
dc.contributor.authorNúñez, Alejandra
dc.contributor.authorMéndez-Bauer, Luján
dc.contributor.authorÑaupari-Villasante, Romina
dc.contributor.authorde Souza, Jullian Josnei
dc.contributor.authorBuvinic, Sonja
dc.contributor.authorDávila-Sánchez, Andrés
dc.contributor.authorFernandez, Eduardo
dc.contributor.authorLoguércio, Alessandro Dourado
dc.date.accessioned2022-02-22T13:02:46Z
dc.date.available2022-02-22T13:02:46Z
dc.date.issued2022-04
dc.identifier10.1016/j.ijadhadh.2022.103107
dc.identifier.issn01437496
dc.identifier.urihttps://hdl.handle.net/20.500.12728/9913
dc.description.abstractThe present study aims to evaluate antimicrobial activity, cytotoxicity, as well as resin-dentine microtensile bond strength, nanoleakage and degree of conversion of six universal adhesives, on sound and caries-affected dentine after 18 months. The adhesives Prime&Bond Active (PBA), Scotchbond Universal (SBU), Tetric N-Bond Universal (TNU), Ambar Universal (AMU), Clearfil Universal Bond Quick (CUQ) and One Coat 7 Universal (OCU) were used. Antimicrobial activity was evaluated against Streptococcus mutans. For cytotoxicity, methyltetrazolium assay was used, after 24 h exposure of osteoblast-like cells line to the adhesive's dilution of 1, 0.1, and 0.01 v/v%. After, the adhesives were applied in etch-and-rinse or self-etch strategies on sound or caries-affected dentine surfaces, resin composites restorations were constructed. Then, the specimens were sectioned to obtain sticks to be evaluated in microtensile bond strength and nanoleakage after 24 h and 18 months, and degree of conversion after 24 h. ANOVA and Tukey's test were applied (α = 0.05). For antimicrobial activity, CUQ showed higher values than all adhesives. For cytotoxicity, the PBA, AMU, CUQ and OCU adhesives presented cytotoxicity in different dilutions. For microtensile bond strength, OCU presented the lowest values, regardless of time, dentine or strategy. For nanoleakage, differences were observed among adhesives depending on time, dentine or strategy. For degree of conversion, TNU presented the highest values, while PBA and OCU presented the lowest values. Worst values of microtensile bond strength and nanoleakage were always obtained in caries-affected dentine after 18 months. Thus, not all universal adhesives behave the same in terms of antimicrobial activity and cytotoxicity. However, the majority showed worst results when applied in caries-affected dentine, mainly after 18 months. Clinical relevance. Universal adhesive systems may have differences in their biological and adhesive properties, both on sound, but mainly in caries-affected dentine after 18 months.es_ES
dc.language.isoenes_ES
dc.publisherElsevier Ltdes_ES
dc.subjectDentinees_ES
dc.subjectHybrid layeres_ES
dc.subjectMechanical properties of adhesiveses_ES
dc.subjectNanoleakagees_ES
dc.subjectUniversal adhesive systemes_ES
dc.titleIn vitro biological and adhesive properties of universal adhesive systems on sound and caries-affected dentine: 18 monthses_ES
dc.typeArticlees_ES


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