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Sex-Dependent Changes of miRNA Levels in the Hippocampus of Adrenalectomized Rats following Acute Corticosterone Administration
dc.contributor.author | Corrales, Wladimir | |
dc.contributor.author | Silva, Juan Pablo | |
dc.contributor.author | Parra, Claudio S. | |
dc.contributor.author | Olave, Felipe Antonio | |
dc.contributor.author | Aguayo, Felipe Ignacio | |
dc.contributor.author | Román-Albasini, Luciano | |
dc.contributor.author | Aliaga, Esteban Enrique | |
dc.contributor.author | Venegas-Zamora, Leslye | |
dc.contributor.author | Ávalos, Ana María | |
dc.contributor.author | Rojas, Paulina Soledad | |
dc.contributor.author | Maracaja-Coutinho, Vinicius | |
dc.contributor.author | Oakley, Robert H. | |
dc.date.accessioned | 2021-09-10T04:39:54Z | |
dc.date.available | 2021-09-10T04:39:54Z | |
dc.date.issued | 2021-08-18 | |
dc.identifier | 10.1021/acschemneuro.0c00762 | |
dc.identifier.issn | 19487193 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12728/9519 | |
dc.description.abstract | We explored sex-biased effects of the primary stress glucocorticoid hormone corticosterone on the miRNA expression profile in the rat hippocampus. Adult adrenalectomized (ADX) female and male rats received a single corticosterone (10 mg/kg) or vehicle injection, and after 6 h, hippocampi were collected for miRNA, mRNA, and Western blot analyses. miRNA profiling microarrays showed a basal sex-biased miRNA profile in ADX rat hippocampi. Additionally, acute corticosterone administration triggered a sex-biased differential expression of miRNAs derived from genes located in several chromosomes and clusters on the X and 6 chromosomes. Putative promoter analysis unveiled that most corticosterone-responsive miRNA genes contained motifs for either direct or indirect glucocorticoid actions in both sexes. The evaluation of transcription factors indicated that almost 50% of miRNA genes sensitive to corticosterone in both sexes was under glucocorticoid receptor regulation. Transcription factor-miRNA regulatory network analyses identified several transcription factors that regulate, activate, or repress miRNA expression. Validated target mRNA analysis of corticosterone-responsive miRNAs showed a more complex miRNA-mRNA interaction network in males compared to females. Enrichment analysis revealed that several hippocampal-relevant pathways were affected in both sexes, such as neurogenesis and neurotrophin signaling. The evaluation of selected miRNA targets from these pathways displayed a strong sex difference in the hippocampus of ADX-vehicle rats. Corticosterone treatment did not change the levels of the miRNA targets and their corresponding tested proteins. Our data indicate that corticosterone exerts a sex-biased effect on hippocampal miRNA expression, which may engage in sculpting the basal sex differences observed at higher levels of hippocampal functioning. | es_ES |
dc.language.iso | en | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.subject | Adrenalectomy | es_ES |
dc.subject | corticosterone | es_ES |
dc.subject | glucocorticoid receptors | es_ES |
dc.subject | hippocampus | es_ES |
dc.subject | miRNAs | es_ES |
dc.subject | sex bias | es_ES |
dc.title | Sex-Dependent Changes of miRNA Levels in the Hippocampus of Adrenalectomized Rats following Acute Corticosterone Administration | es_ES |
dc.type | Article | es_ES |