Changes in the cell wall components produced by exogenous abscisic acid treatment in strawberry fruit
Autor
Castro, Ricardo I.
Gonzalez-Feliu, Ana
Valenzuela-Riffo, Felipe
Parra-Palma, Carolina
Morales-Quintana, Luis
Resumen
Abstract: Fruit development and ripening are controlled by multiple plant hormones; for strawberries, recent evidence supports the role of abscisic acid (ABA) as a promoter of fruit ripening. Fruit softening during ripening is mainly a consequence of the solubilization and depolymerization of cell wall components mediated by the action of a complex set of enzymes and proteins. In the present work, we performed a comparative study (ABA-treatment vs. control) of the changes in the physiological properties of the cell wall-associated polysaccharide contents of strawberry fruit (Fragaria x ananassa ‘Camarosa’) via analysis of thermogravimetry (TG) combined with analysis of mRNA abundance, enzymatic activity and physiological characteristics. ‘Camarosa’ did not show a decline in the fruit firmness at 48 h post-treatment; however, we observed changes in cell wall stability based on the TG and differential thermogravimetric (DTG) analysis curves, which demonstrated the degradation of the cell wall polymers after ABA hormone treatment for 48 h, principally for hemicellulose polymers. Additionally, DTG analysis showed that dried samples derived from the treatment of the fruit with the ABA biosynthesis inhibitor fluridone maintained the same thermal stability as the control samples. Finally, the existence of a relationship between thermal stability, transcriptional analysis and enzymatic activity after hormone treatment was demonstrated, which provides the basis for a model for understanding the changes in the cell wall polymers of F. x ananassa mediated by the ABA hormone during fruit ripening. Graphic abstract: [Figure not available: see fulltext.]
Colecciones
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Article
Effect of exogenous auxin treatment on cell wall polymers of strawberry fruit (2021)
Castro, Ricardo I.; González-Feliu, Ana; Muñoz-Vera, Marcelo; Valenzuela-Riffo, Felipe; Parra-Palma, Carolina; Morales-Quintana, Luis (MDPI AG, 2021-06-02)The role of auxin in the fruit-ripening process during the early developmental stages of commercial strawberry fruits (Fragaria x ananassa) has been previously described, with auxin production occurring in achenes and ... -
Article
Characterization of cell wall modification through thermogravimetric analysis during ripening of Chilean strawberry (Fragaria chiloensis) fruit (2021)
Castro, Ricardo I.; Muñoz-Vera, Marcelo; Parra-Palma, Carolina; Valenzuela-Riffo, Felipe; Figueroa, Carlos R.; Morales-Quintana, Luis (Springer Science and Business Media B.V., 2021)Fragaria chiloensis (Chilean strawberry) fruit has been described as a fruit with excellent organoleptic properties, highlighting its flavor and aroma. However, the fruit has a high softening rate. Fruit softening during ... -
Article
Cell wall-related enzymatic activities and transcriptional profiles in four strawberry (Fragaria x ananassa) cultivars during fruit development and ripening (2020)
Ramos P.; Parra-Palma C.; Figueroa C.R.; Zuñiga P.E.; Valenzuela-Riffo F.; Gonzalez J.; Gaete-Eastman C.; Morales-Quintana L. (Elsevier B.V., 2018)