Landscape of genome-wide dna methylation of colorectal cancer metastasis
Autor
Ili, Carmen
Buchegger, Kurt
Demond, Hannah
Castillo-Fernandez, Juan
Kelsey, Gavin D.
Zanella, Louise
Abanto, Michel
Riquelme, Ismael
López, Jaime
Viscarra, Tamara
García, Patricia C.
Bellolio, Enrique
Saavedra, David
Brebi, Priscilla
Resumen
Colorectal cancer is a heterogeneous disease caused by both genetic and epigenetics factors. Analysing DNA methylation changes occurring during colorectal cancer progression and metastasis formation is crucial for the identification of novel epigenetic markers of patient prognosis. Genome-wide methylation sequencing of paired samples of colon (normal adjacent, primary tumour and lymph node metastasis) showed global hypomethylation and CpG island (CGI) hypermethylation of primary tumours compared to normal. In metastasis we observed high global and non-CGI regions methylation, but lower CGI methylation, compared to primary tumours. Gene ontology analysis showed shared biological processes between hypermethylated CGIs in metastasis and primary tumours. After complementary analysis with The Cancer Genome Atlas (TCGA) cohort, FIGN, HTRA3, BDNF, HCN4 and STAC2 genes were found associated with poor survival. We mapped the methylation landscape of colon normal tissues, primary tumours and lymph node metastasis, being capable of identified methylation changes throughout the genome. Furthermore, we found five genes with potential for methylation biomarkers of poor prognosis in colorectal cancer patients.
Colecciones
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Article
Genetic patterns found in the nuclear localization signals (Nlss) associated with ebv-1 and ebv-2 provide new insights into their contribution to different cell-type specificities (2021)
Zanella, Louise; Reyes, María Elena; Riquelme, Ismael; Abanto, Michel; León, Daniela; Viscarra, Tamara; Ili, Carmen; Brebi, Priscilla (MDPI AG, 2021-06-01)The Epstein–Barr virus (EBV) is a globally dispersed pathogen involved in several human cancers of B-cell and non-B-cell origin. EBV has been classified into EBV-1 and EBV-2, which have differences in their transformative ... -
Article
Epigallocatechin gallate enhances MAL-PDT cytotoxic effect on PDT-resistant skin cancer squamous cells (2022)
León, Daniela; Buchegger, Kurt; Silva, Ramón; Riquelme, Ismael; Viscarra, Tamara; Mora-Lagos, Bárbara; Zanella, Louise; Schafer, Fabiola; Kurachi, Cristina; Roa, Juan Carlos; ... (International journal of molecular sciences, 2020-01)Photodynamic therapy (PDT) has been used to treat certain types of non-melanoma skin cancer with promising results. However, some skin lesions have not fully responded to this treatment, suggesting a potential PDT-resistant ... -
Article
Exploring the Genetic Diversity of Epstein–Barr Virus among Patients with Gastric Cancer in Southern Chile (2024)
Reyes, María Elena; Zanella, Louise; Riquelme, Ismael; Buchegger, Kurt; Mora-Lagos, Bárbara; Guzmán, Pablo; García, Patricia; Roa, Juan C.; Ili, Carmen Gloria; Brebi, Priscilla (Multidisciplinary Digital Publishing Institute (MDPI), 2023)The Epstein–Barr virus (EBV) has been associated with gastric cancer (GC), one of the deadliest malignancies in Chile and the world. Little is known about Chilean EBV strains. This study aims to investigate the frequency ...