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Amplification of tlh gene in other Vibrionaceae specie by specie-specific multiplex PCR of Vibrio parahaemolyticus

dc.contributor.authorYáñez R.
dc.contributor.authorBastías R.
dc.contributor.authorHiguera G.
dc.contributor.authorSalgado O.
dc.contributor.authorKatharios P.
dc.contributor.authorRomero J.
dc.contributor.authorEspejo R.
dc.contributor.authorGarcía K.
dc.date.accessioned2020-09-02T22:30:38Z
dc.date.available2020-09-02T22:30:38Z
dc.date.issued2015
dc.identifier10.1016/j.ejbt.2015.09.007
dc.identifier.citation18, 6, 459-463
dc.identifier.issn07173458
dc.identifier.urihttps://hdl.handle.net/20.500.12728/6658
dc.descriptionBackground: The surveillance of Vibrio parahaemolyticus in the Chilean coast has been mainly performed by multiplex PCR amplification of three different hemolysin genes, which are specie-specific virulence factors. These genes are also employed in the determination of V. parahaemolyticus pathogenic load in seafood and for characterization of pathogenic strains associated to diarrhea cases in human. During environmental surveillance that we performed every summer, we occasionally observed a thermolabile hemolysin (tlh) PCR product of a slightly smaller size than expected, which was coincident with low loads of V. parahaemolyticus in the environment. In order to understand this observation, we probed the specificity of tlh primers for detection of V. parahaemolyticus at different bacterial loads and DNA concentrations. Results: Primers used for detection of V. parahaemolyticus specific tlh amplified a slightly smaller tlh gene, which is found in Vibrio alginolyticus and other related strains. These amplicons were observed when V. parahaemolyticus was absent or in undetectable loads in the environment. Conclusions: Surveillance of V. parahaemolyticus using tlh primers can be imprecise because amplification of a V. parahaemolyticus specific marker in V. alginolyticus and other related strains occurs. This situation complicates potentially the estimation of bacterial load in seafood, because do not ensure the correct identification of V. parahaemolyticus when his load is low. Additionally, it could complicate the tracking of outbreaks of V. parahaemolyticus infections, considering the genetic markers used would not be specie-specific. © 2015 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserved.
dc.language.isoen
dc.publisherElectronic Journal of Biotechnology
dc.subjectHemolysin
dc.subjectMultiplex PCR
dc.subjectPathogen surveillance
dc.subjectVibrio parahaemolyticus
dc.subjectVirulence factor
dc.subjectBlood
dc.subjectGenes
dc.subjectMonitoring
dc.subjectMultiplexing
dc.subjectEnvironmental surveillance
dc.subjectHemolysin
dc.subjectMultiplex pcr
dc.subjectPathogenic strains
dc.subjectV. parahaemolyticus
dc.subjectVibrio alginolyticus
dc.subjectVibrio parahaemolyticus
dc.subjectVirulence factors
dc.subjectPolymerase chain reaction
dc.subjectbacterial DNA
dc.subjecthemolysin
dc.subjectamplicon
dc.subjectArticle
dc.subjectbacterial gene
dc.subjectbacterial load
dc.subjectbacterial strain
dc.subjectbacterium detection
dc.subjectbacterium examination
dc.subjectbacterium identification
dc.subjectcontrolled study
dc.subjectDNA sequence
dc.subjectgene amplification
dc.subjectmultiplex polymerase chain reaction
dc.subjectnonhuman
dc.subjectphylogeny
dc.subjecttlh gene
dc.subjectVibrio parahaemolyticus
dc.titleAmplification of tlh gene in other Vibrionaceae specie by specie-specific multiplex PCR of Vibrio parahaemolyticus
dc.typeArticle


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