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Acid-sensing ion channels (ASICs) 2 and 4.2 are expressed in the retina of the adult zebrafish

dc.contributor.authorViña E.
dc.contributor.authorParisi V.
dc.contributor.authorSánchez-Ramos C.
dc.contributor.authorCabo R.
dc.contributor.authorGuerrera M.C.
dc.contributor.authorQuirós L.M.
dc.contributor.authorGermanà A.
dc.contributor.authorVega J.A.
dc.contributor.authorGarcía-Suárez O.
dc.date.accessioned2020-09-02T22:30:27Z
dc.date.available2020-09-02T22:30:27Z
dc.date.issued2015
dc.identifier10.1007/s00441-014-2084-5
dc.identifier.citation360, 2, 223-231
dc.identifier.issn0302766X
dc.identifier.urihttps://hdl.handle.net/20.500.12728/6595
dc.descriptionAcid-sensing ion channels (ASICs) are H+-gated, voltage-insensitive cation channels involved in synaptic transmission, mechanosensation and nociception. Different ASICs have been detected in the retina of mammals but it is not known whether they are expressed in adult zebrafish, a commonly used animal model to study the retina in both normal and pathological conditions. We study the expression and distribution of ASIC2 and ASIC4 in the retina of adult zebrafish and its regulation by light using PCR, in situ hybridization, western blot and immunohistochemistry. We detected mRNA encoding zASIC2 and zASIC4.2 but not zASIC4.1. ASIC2, at the mRNA or protein level, was detected in the outer nuclear layer, the outer plexiform layer, the inner plexiform layer, the retinal ganglion cell layer and the optic nerve. ASIC4 was expressed in the photoreceptors layer and to a lesser extent in the retinal ganglion cell layer. Furthermore, the expression of both ASIC2 and ASIC4.2 was down-regulated by light and darkness. These results are the first demonstration that ASIC2 and ASIC4 are expressed in the adult zebrafish retina and suggest that zebrafish could be used as a model organism for studying retinal pathologies involving ASICs. © 2015, Springer-Verlag Berlin Heidelberg.
dc.language.isoen
dc.publisherSpringer Verlag
dc.subjectAcid-sensing ion channels
dc.subjectLight
dc.subjectRetina
dc.subjectZebrafish
dc.subjectacid sensing ion channel
dc.subjecteye protein
dc.subjectzebrafish protein
dc.subjectanimal
dc.subjectbiosynthesis
dc.subjectcytology
dc.subjectgene expression regulation
dc.subjectmetabolism
dc.subjectphysiology
dc.subjectretina
dc.subjectzebra fish
dc.subjectAcid Sensing Ion Channels
dc.subjectAnimals
dc.subjectEye Proteins
dc.subjectGene Expression Regulation
dc.subjectRetina
dc.subjectZebrafish
dc.subjectZebrafish Proteins
dc.titleAcid-sensing ion channels (ASICs) 2 and 4.2 are expressed in the retina of the adult zebrafish
dc.typeArticle


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