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Identification of volatile biomarkers of Giardia duodenalis infection in children with persistent diarrhoea

dc.contributor.authorUbeda C.
dc.contributor.authorLepe-Balsalobre E.
dc.contributor.authorAriza-Astolfi C.
dc.contributor.authorUbeda-Ontiveros J.M.
dc.date.accessioned2020-09-02T22:29:27Z
dc.date.available2020-09-02T22:29:27Z
dc.date.issued2019
dc.identifier10.1007/s00436-019-06433-4
dc.identifier.citation118, 11, 3139-3147
dc.identifier.issn09320113
dc.identifier.urihttps://hdl.handle.net/20.500.12728/6458
dc.descriptionCurrently, chronic diarrhoea syndrome in children is a very common pathology whose aetiology is sometimes difficult to identify. Methodologies for the diagnosis of infections have diversified, and gas chromatography/mass spectrometry (GC/MS) is a very useful tool. The aim of this study was to identify volatile biomarkers of the presence of Giardia duodenalis in the faeces of patients with chronic diarrhoea (with and without giardiasis) using static headspace extraction followed by GC/MS. The analysis of the volatiles extracted from the headspace had enough sensitivity to detect differences in the volatile profiles in the faeces of the patients with and without Giardia duodenalis infection and discriminate between them. Dimethyl disulphide and trisulphide were found in the faeces of patients without giardiasis but not in the faeces of patients with G. duodenalis. Finally, three possible biomarkers, acetic acid, 1,4-dimethoxy-2,3-butanediol and 1,3-dimethoxy-2-propanol, were proposed to identify patients with giardiasis; these compounds were not present in the patients without the parasite. Multivariate analysis revealed that principal component 1 separated the stool samples according to the presence of infection by G. duodenalis despite the inter-individual variability in biological specimens such as faeces. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
dc.language.isoen
dc.publisherSpringer Verlag
dc.subjectBiomarkers
dc.subjectChronic diarrhoea
dc.subjectGas chromatography
dc.subjectGiardia duodenalis
dc.subjectPersistent diarrhoea
dc.subjectVolatile compounds
dc.subject1,3 dimethoxy 2 propanol
dc.subject1,4 dimethoxy 2,3 butanediol
dc.subjectacetic acid
dc.subjectaldehyde
dc.subjectamide
dc.subjectbiological marker
dc.subjectbutanoate
dc.subjectbutyric acid ethyl ester
dc.subjecthexanoic acid
dc.subjectisobutanol
dc.subjectmethyl heptanoate
dc.subjectpara cymene
dc.subjectpentanoate
dc.subjectpentanol
dc.subjectpropyl butanoate
dc.subjectsulfur
dc.subjectterpene
dc.subjectunclassified drug
dc.subjectvolatile agent
dc.subjectbiological marker
dc.subjectArticle
dc.subjectchild
dc.subjectchronic diarrhea
dc.subjectclinical article
dc.subjectdiarrhea
dc.subjectfeces
dc.subjectfemale
dc.subjectgiardiasis
dc.subjectheadspace extraction
dc.subjecthost
dc.subjecthuman
dc.subjectintestine flora
dc.subjectmale
dc.subjectmass fragmentography
dc.subjectprincipal component analysis
dc.subjectpriority journal
dc.subjectanimal
dc.subjectGiardia intestinalis
dc.subjectgiardiasis
dc.subjectmultivariate analysis
dc.subjectparasitology
dc.subjectpreschool child
dc.subjectAnimals
dc.subjectBiomarkers
dc.subjectChild
dc.subjectChild, Preschool
dc.subjectDiarrhea
dc.subjectFeces
dc.subjectFemale
dc.subjectGas Chromatography-Mass Spectrometry
dc.subjectGiardia lamblia
dc.subjectGiardiasis
dc.subjectHumans
dc.subjectMale
dc.subjectMultivariate Analysis
dc.titleIdentification of volatile biomarkers of Giardia duodenalis infection in children with persistent diarrhoea
dc.typeArticle


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