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Antifungal activity of proteolytic fraction (P1G10) from (Vasconcellea cundinamarcensis) latex inhibit cell growth and cell wall integrity in Botrytis cinerea

dc.contributor.authorTorres-Ossandón M.J.
dc.contributor.authorVega-Gálvez A.
dc.contributor.authorSalas C.E.
dc.contributor.authorRubio J.
dc.contributor.authorSilva-Moreno E.
dc.contributor.authorCastillo L.
dc.date.accessioned2020-09-02T22:29:20Z
dc.date.available2020-09-02T22:29:20Z
dc.date.issued2019
dc.identifier10.1016/j.ijfoodmicro.2018.08.027
dc.identifier.citation289, , 7-16
dc.identifier.issn01681605
dc.identifier.urihttps://hdl.handle.net/20.500.12728/6421
dc.descriptionThe aim of this study was to determine the antifungal activity of the proteolytic fraction P1G10 from Vasconcellea cundinamarcencis (ex-Carica candamarcensis) against Botrytis cinerea, the causative agent of pre- and postharvest damaging disease in fruit and vegetables. The survival of B. cinerea at different concentrations of P1G10 showed that 1 mg/mL inhibited 50% of mycelium growth after 72 h incubation. The kinetic of growth inhibition fits the Weibull distribution function, and the data was confirmed by the IC50 survival assay. The study shows that P1G10 inhibits conidia germination and germ tube elongation of B. cinerea relative to untreated conidia. Hypersensitivity to cell wall-perturbing agents (Calcofluor white and Congo red) was observed in mycelium cells treated with P1G10. In addition, P1G10 exhibited inhibitory effect on the adhesion of conidia, provoked alterations in membrane integrity and induced production of reactive oxygen species accompanied by cellular damage. Our results highlight the effect of P1G10 on mycelium growth, cell wall alterations, membrane integrity and adhesion. P1G10 emerges as promising antifungal to control disease causing agents in the food agroindustry. © 2018
dc.language.isoen
dc.publisherElsevier B.V.
dc.subjectAntifungal activity
dc.subjectBotrytis cinerea
dc.subjectP1G10
dc.subjectProteolytic enzymes
dc.subjectVasconcellea cundinamarcencis
dc.subjectlatex
dc.subjectplant extract
dc.subjectreactive oxygen metabolite
dc.subjectunclassified drug
dc.subjectVasconcellea cundinamarcencis extract
dc.subjectantifungal agent
dc.subjectlatex
dc.subjectplant extract
dc.subjectantifungal activity
dc.subjectArticle
dc.subjectBotrytis cinerea
dc.subjectcell adhesion
dc.subjectcell damage
dc.subjectcell growth
dc.subjectcell survival
dc.subjectconidium
dc.subjectcontrolled study
dc.subjectfungal cell wall
dc.subjectfungal membrane
dc.subjectgene expression
dc.subjectgermination
dc.subjectIC50
dc.subjectmycelial growth
dc.subjectmycelium
dc.subjectnonhuman
dc.subjectplant
dc.subjectVasconcellea cundinamarcencis
dc.subjectBotrytis
dc.subjectCarica
dc.subjectcell wall
dc.subjectchemistry
dc.subjectdrug effect
dc.subjectfood control
dc.subjectfruit
dc.subjectfungus spore
dc.subjectgrowth, development and aging
dc.subjectmicrobiology
dc.subjectprotein degradation
dc.subjectAntifungal Agents
dc.subjectBotrytis
dc.subjectCarica
dc.subjectCell Wall
dc.subjectFood Microbiology
dc.subjectFruit
dc.subjectLatex
dc.subjectMycelium
dc.subjectPlant Extracts
dc.subjectProteolysis
dc.subjectSpores, Fungal
dc.titleAntifungal activity of proteolytic fraction (P1G10) from (Vasconcellea cundinamarcensis) latex inhibit cell growth and cell wall integrity in Botrytis cinerea
dc.typeArticle


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