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Towards opto-magnetic physiology: Concepts and pitfalls of of MRI

dc.contributor.authorSchwalm M.
dc.contributor.authorJubal E.R.
dc.contributor.authorStroh A.
dc.date.accessioned2020-09-02T22:28:09Z
dc.date.available2020-09-02T22:28:09Z
dc.date.issued2018
dc.identifier10.1007/978-1-4939-7417-7_13
dc.identifier.citation133, , 257-276
dc.identifier.issn08932336
dc.identifier.urihttps://hdl.handle.net/20.500.12728/6225
dc.descriptionOptogenetic functional magnetic resonance imaging (ofMRI) represents the combination of optogenetic modulation of neural circuits with high-field fMRI. ofMRI enables the monitoring of neural activity across the entire brain, while precisely controlling the activation of specific neuronal elements within the neural circuit, defined by their genetic identity, location of somata, and axonal projection targets. Those exclusive features of ofMRI open a plethora of new possibilities for the in vivo characterization of neural networks, simultaneously at local and global scale. In addition, recent advances in optical readouts of intracellular Ca2+ within optogenetic fMRI experiments led to all-optical interrogations within the scanner. Here, we provide a guide to applying optogenetics and optical recordings in the setting of fMRI experiments. In addition, we devise a straight forward control experiment addressing the discrimination of specific network activation through the excitation of opsins from unspecific heat effects leading to an apparent BOLD effect. Lastly, we propose a method to estimate the scope of optogenetic network modulation by combining the estimations of the number of opsin-expressing neurons and light spreading in brain tissue. © Springer Science+Business Media LLC 2018.
dc.language.isoen
dc.publisherHumana Press Inc.
dc.subjectBOLD fMRI
dc.subjectCa2+ recordings
dc.subjectOfMRI
dc.subjectOptical recordings
dc.subjectOptogenetics
dc.subjectopsin
dc.subjectBOLD signal
dc.subjecthemodynamics
dc.subjecthuman
dc.subjectimage processing
dc.subjectmagnetic field
dc.subjectnerve cell
dc.subjectnerve cell network
dc.subjectnerve cell stimulation
dc.subjectneuromodulation
dc.subjectnonhuman
dc.subjectnuclear magnetic resonance imaging
dc.subjectoptogenetic functional magnetic resonance imaging
dc.subjectoptogenetics
dc.subjectpriority journal
dc.titleTowards opto-magnetic physiology: Concepts and pitfalls of of MRI
dc.typeBook Chapter


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