Quantification of collagen fibers in canine uteri treated with medroxyprogesterone acetate

Salinas P.; Miglino M.A.; del Sol M.

dc.contributor.author	Salinas P.
dc.contributor.author	Miglino M.A.
dc.contributor.author	del Sol M.
dc.date.accessioned	2020-09-02T22:27:40Z
dc.date.available	2020-09-02T22:27:40Z
dc.date.issued	2016
dc.identifier	10.1590/S0100-736X2016001200014
dc.identifier.citation	36, 12, 1221-1226
dc.identifier.issn	0100736X
dc.identifier.uri	https://hdl.handle.net/20.500.12728/6158
dc.description	Collagen plays essential roles in remodeling uterine tissue during decidualization, implantation, pregnancy and involution. To understand whether the progestational agent medroxyprogesterone acetate (MPA) can modify the organization and deposit of collagen in the uteri of normal bitches (Canis Tlupus familiaris), we assessed uterine tissues by histochemistry. Uteri were grouped as: nulliparous (n=11), multiparous (n=11) and treated with MPA (n=11; nulliparous with two treatments; 5mg/kg; i.m.). The amount, location and birefringence of interstitial collagen types I and III in the fold and base of the endometrial stroma and the myometrial muscular layers were studied on sections stained with Picrosirius Red by polarized light microscopy and evaluated by ANOVA. No differences were observed in the endometrium. In the myometrium, differences were observed in collagen type I between MPA-treated and nulliparous uteri vs. multiparous (p<0.05), and differences in collagen type III between nulliparous and multiparous uteri vs. MPA-treated (p=0.0001). In conclusion, two doses of MPA had no significant effect on the investigated collagens in the extracellular matrix.
dc.language.iso	en
dc.publisher	Colegio Brasileiro de Patologia Animal
dc.subject	Canis lupus familiaris
dc.subject	Collagen
dc.subject	Medroxyprogesterone acetate
dc.subject	Picrosirius red
dc.subject	Uterus
dc.title	Quantification of collagen fibers in canine uteri treated with medroxyprogesterone acetate
dc.type	Article