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Serotype a of Aggregatibacter actinomycetemcomitans down-regulates the increased serotype b-induced cytokine and chemokine production in dendritic cells

dc.contributor.authorRojas L.
dc.contributor.authorMelgar-Rodríguez S.
dc.contributor.authorDíaz-Zúñiga J.
dc.contributor.authorAlvarez C.
dc.contributor.authorMonasterio G.
dc.contributor.authorRojas C.
dc.contributor.authorCarvajal P.
dc.contributor.authorVernal R.
dc.date.accessioned2020-09-02T22:27:09Z
dc.date.available2020-09-02T22:27:09Z
dc.date.issued2018
dc.identifier10.1016/j.archoralbio.2018.06.010
dc.identifier.citation93, , 155-162
dc.identifier.issn00039969
dc.identifier.urihttps://hdl.handle.net/20.500.12728/6069
dc.descriptionObjective: In Aggregatibacter actinomycetemcomitans, different serotypes have been described based on LPS antigenicity. Mixed infection with the different A. actinomycetemcomitans serotypes is frequent in periodontitis patients; accordingly, the role of this bacterial species in the pathogenesis of periodontitis may differ depending whether patients or periodontal lesions harbour one or more of the A. actinomycetemcomitans serotypes. We hypothesized that different combinations of these serotypes could be associated with distinct host responses and hence different inflammatory patterns. This investigation was aimed to assess whether the increased immuno-stimulatory potential attributed to the serotype b of A. actinomycetemcomitans on immune cells is able to be modified during co-infection with other A. actinomycetemcomitans serotypes. Methods: Dendritic cells (DCs) were obtained from healthy subjects and stimulated with the different A. actinomycetemcomitans serotypes or their purified LPS using the following stimulatory conditions: serotype a, b, or c, and the combinations a+b, a+c, b+c, or a+b+c. The cytokine, CCR, and CCL levels were quantified by qPCR and ELISA. Results: Higher levels of cytokines, CCRs, and CCLs were induced when DCs were stimulated with the serotype b of A. actinomycetemcomitans compared with the same cells stimulated with the other serotypes. When DCs were co-infected, these levels decreased in comparison with the serotype b-stimulation alone, in particular when the serotype a was present in the mixed infection. Conclusions: The increased immuno-stimulatory potential attributed to the serotype b was modified when DCs were co-infected with other A. actinomycetemcomitans serotypes, in particular, when the serotype a was present, the DC response diminished. © 2018 Elsevier Ltd
dc.language.isoen
dc.publisherElsevier Ltd
dc.subjectA. actinomycetemcomitans
dc.subjectChemokine receptors
dc.subjectChemokines
dc.subjectCytokines
dc.subjectSerotypes
dc.subjectchemokine
dc.subjectcytokine
dc.subjectlipopolysaccharide
dc.subjectadult
dc.subjectAggregatibacter actinomycetemcomitans
dc.subjectdendritic cell
dc.subjectdown regulation
dc.subjectenzyme linked immunosorbent assay
dc.subjectfemale
dc.subjecthuman
dc.subjectimmunology
dc.subjectmale
dc.subjectpolymerase chain reaction
dc.subjectserotype
dc.subjectAdult
dc.subjectAggregatibacter actinomycetemcomitans
dc.subjectChemokines
dc.subjectCytokines
dc.subjectDendritic Cells
dc.subjectDown-Regulation
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFemale
dc.subjectHumans
dc.subjectLipopolysaccharides
dc.subjectMale
dc.subjectPolymerase Chain Reaction
dc.subjectSerogroup
dc.titleSerotype a of Aggregatibacter actinomycetemcomitans down-regulates the increased serotype b-induced cytokine and chemokine production in dendritic cells
dc.typeArticle


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