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Inhibitory effect of serotype a of Aggregatibacter actinomycetemcomitans on the increased destructive potential of serotype b
dc.contributor.author | Rojas L. | |
dc.contributor.author | Melgar-Rodríguez S. | |
dc.contributor.author | Díaz-Zúñiga J. | |
dc.contributor.author | Alvarez C. | |
dc.contributor.author | Monasterio G. | |
dc.contributor.author | Rojas C. | |
dc.contributor.author | Cafferata E.A. | |
dc.contributor.author | Hernández M. | |
dc.contributor.author | Cortéz C. | |
dc.contributor.author | Carvajal P. | |
dc.contributor.author | Vernal R. | |
dc.date.accessioned | 2020-09-02T22:27:09Z | |
dc.date.available | 2020-09-02T22:27:09Z | |
dc.date.issued | 2020 | |
dc.identifier | 10.1111/odi.13237 | |
dc.identifier.citation | 26, 2, 409-418 | |
dc.identifier.issn | 1354523X | |
dc.identifier.uri | https://hdl.handle.net/20.500.12728/6068 | |
dc.description | Objective: The serotype b of Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) induces higher cytokine production in dendritic cells (DCs) compared with the other serotypes. However, this increased immunostimulatory potential was modified when DCs were co-infected with the other A. actinomycetemcomitans serotypes. This study aimed to analyze whether the production of interferon gamma (IFN-γ), C-reactive protein (CRP), matrix metalloproteinase (MMP)-2, and MMP-9, as well as the activity of osteoclasts, also varies when DCs are co-infected with the A. actinomycetemcomitans serotypes. Materials and Methods: Human DCs were stimulated with the A. actinomycetemcomitans serotypes using the following stimulatory conditions: serotype a/b/c/a+b/a+c/b+c/a+b+c. The IFN-γ, CRP, and MMP-2 levels were quantified by ELISA. The active form of MMP-9 was quantified using fluorescent functional assays. The MMP-2 gelatinolytic activity was identified by zymogram. The osteoclast activity was determined by quantifying the TRAP expression and resorption-pit formation using cytochemistry and osteoassays. Results: Higher levels of IFN-γ, CRP, MMP-2, MMP-9, and osteoclast activity were detected when DCs were stimulated with the serotype b of A. actinomycetemcomitans compared with the others. This increased immunostimulatory potential attributed to serotype b diminished when DCs were co-infected with the serotype a. Conclusions: This study provides new insights into the virulence of A. actinomycetemcomitans and reveals important differences in the immunostimulatory and pro-destructive potential among its serotypes. © 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved | |
dc.language.iso | en | |
dc.publisher | Blackwell Publishing Ltd | |
dc.subject | A. actinomycetemcomitans | |
dc.subject | bone resorption | |
dc.subject | cytokines | |
dc.subject | matrix metalloproteinases | |
dc.subject | osteoclasts | |
dc.subject | serotypes | |
dc.subject | C reactive protein | |
dc.subject | gamma interferon | |
dc.subject | gelatinase A | |
dc.subject | gelatinase B | |
dc.subject | C reactive protein | |
dc.subject | gamma interferon | |
dc.subject | gelatinase A | |
dc.subject | gelatinase B | |
dc.subject | MMP2 protein, human | |
dc.subject | MMP9 protein, human | |
dc.subject | Aggregatibacter actinomycetemcomitans | |
dc.subject | Article | |
dc.subject | bacterial virulence | |
dc.subject | controlled study | |
dc.subject | cytochemistry | |
dc.subject | dendritic cell | |
dc.subject | enzyme linked immunosorbent assay | |
dc.subject | human | |
dc.subject | human cell | |
dc.subject | nonhuman | |
dc.subject | osteoclast activity | |
dc.subject | osteolysis | |
dc.subject | priority journal | |
dc.subject | protein expression | |
dc.subject | serotype | |
dc.subject | zymography | |
dc.subject | Aggregatibacter actinomycetemcomitans | |
dc.subject | classification | |
dc.subject | metabolism | |
dc.subject | microbiology | |
dc.subject | osteoclast | |
dc.subject | pathogenicity | |
dc.subject | serotype | |
dc.subject | Aggregatibacter actinomycetemcomitans | |
dc.subject | C-Reactive Protein | |
dc.subject | Dendritic Cells | |
dc.subject | Humans | |
dc.subject | Interferon-gamma | |
dc.subject | Matrix Metalloproteinase 2 | |
dc.subject | Matrix Metalloproteinase 9 | |
dc.subject | Osteoclasts | |
dc.subject | Serogroup | |
dc.title | Inhibitory effect of serotype a of Aggregatibacter actinomycetemcomitans on the increased destructive potential of serotype b | |
dc.type | Article |