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A novel “in-feed” delivery platform applied for oral DNA vaccination against IPNV enables high protection in Atlantic salmon (Salmon salar)

dc.contributor.authorReyes M.
dc.contributor.authorRamírez C.
dc.contributor.authorÑancucheo I.
dc.contributor.authorVillegas R.
dc.contributor.authorSchaffeld G.
dc.contributor.authorKriman L.
dc.contributor.authorGonzalez J.
dc.contributor.authorOyarzun P.
dc.date.accessioned2020-09-02T22:26:58Z
dc.date.available2020-09-02T22:26:58Z
dc.date.issued2017
dc.identifier10.1016/j.vaccine.2016.12.013
dc.identifier.citation35, 4, 626-632
dc.identifier.issn0264410X
dc.identifier.urihttps://hdl.handle.net/20.500.12728/6005
dc.descriptionBackground DNA vaccination has emerged as a promising tool against infectious diseases of farmed fish. Oral delivery allows stress-free administration that is ideal for mass immunization and of paramount importance for infectious pancreatic necrosis (IPN) and other viral disease that affect young salmonids and cause economic losses in aquaculture worldwide. Methods We describe the development and in vivo assessment of an “in-feed” formulation strategy for oral immunization with liposomal DNA vaccines, by delivering a vaccine construct coding for an immunogenic region of the VP2 capsid protein. A challenge against IPNV was carried out to determine the vaccine efficacy, by comparing the mortality of pre-smolt Atlantic salmons immunized and non-immunized with the oral vaccine. The antibody response (ELISA) and hematological parameters after immunization were examined, as well as the vaccine effect on the growth and internal structures of fry salmons (histological analysis). The vaccine distribution in the experimental tank after oral administration was investigated by HPLC and PCR amplification. Results The oral vaccine induced detectable levels of VP2-specific antibodies and conferred significant protection following IPNV challenge, with relative percent survivals (RPS) of 58.2%, for single dose (1 mgpDNA/kgfish⋅d), and 66% for double dose (2 mgpDNA/kgfish⋅d). We further provide evidence in favour of the vaccine safety to fish and demonstrated absence of pDNA in the tank water, but presence of vaccine residues in faeces and unconsumed feed sediments (solid wastes). Conclusion The delivery platform for liposomal DNA vaccination via feed was successfully proved against IPNV in Atlantic salmon, showing the oral vaccine to be immunogenic and safe for fish, and providing significant protection after oral administration. The “in-feed” technology for oral DNA vaccination holds potential to be applied against IPNV and other pathogens that currently threaten the aquaculture worldwide. © 2016 Elsevier Ltd
dc.language.isoen
dc.publisherElsevier Ltd
dc.subjectAtlantic salmon
dc.subjectDNA vaccine
dc.subjectDOTAP
dc.subjectFeed
dc.subjectIPNV
dc.subjectLiposome
dc.subjectOral delivery
dc.subjectSalmon salar
dc.subject1,2 dioleoyl 3 trimethylammoniopropane
dc.subjectDNA vaccine
dc.subjectinfectious pancreatic necrosis virus vaccine
dc.subjectprotein VP2
dc.subjectunclassified drug
dc.subjectvirus vaccine
dc.subjectDNA vaccine
dc.subjectdrug carrier
dc.subjectimmunological adjuvant
dc.subjectliposome
dc.subjectvirus antibody
dc.subjectanimal food
dc.subjectanimal tissue
dc.subjectantibody detection
dc.subjectaquaculture
dc.subjectArticle
dc.subjectcontrolled study
dc.subjectDNA immunization
dc.subjectdrug safety
dc.subjecthistology
dc.subjectin vivo study
dc.subjectinfectious pancreatic necrosis
dc.subjectinfectious pancreatic necrosis virus
dc.subjectliposomal delivery
dc.subjectliver
dc.subjectmortality
dc.subjectnonhuman
dc.subjectpancreas
dc.subjectparticle size
dc.subjectpriority journal
dc.subjectprovocation test
dc.subjectSalmo salar
dc.subjectanimal
dc.subjectanimal structures
dc.subjectbirnavirus infection
dc.subjectblood
dc.subjectenzyme linked immunosorbent assay
dc.subjectfish disease
dc.subjectimmunology
dc.subjectinfectious pancreatic necrosis virus
dc.subjectoral drug administration
dc.subjectpathology
dc.subjectsurvival analysis
dc.subjecttreatment outcome
dc.subjectveterinary
dc.subjectAdjuvants, Immunologic
dc.subjectAdministration, Oral
dc.subjectAnimal Structures
dc.subjectAnimals
dc.subjectAntibodies, Viral
dc.subjectBirnaviridae Infections
dc.subjectDrug Carriers
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFish Diseases
dc.subjectInfectious pancreatic necrosis virus
dc.subjectLiposomes
dc.subjectSalmo salar
dc.subjectSurvival Analysis
dc.subjectTreatment Outcome
dc.subjectVaccines, DNA
dc.titleA novel “in-feed” delivery platform applied for oral DNA vaccination against IPNV enables high protection in Atlantic salmon (Salmon salar)
dc.typeArticle


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