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Genetic and phenotypic evidence of the Salmonella enterica serotype Enteritidis human-animal interface in Chile

dc.contributor.authorRetamal P.
dc.contributor.authorFresno M.
dc.contributor.authorDougnac C.
dc.contributor.authorGutierrez S.
dc.contributor.authorGornall V.
dc.contributor.authorVidal R.
dc.contributor.authorVernal R.
dc.contributor.authorPujol M.
dc.contributor.authorBarreto M.
dc.contributor.authorGonzález-Acuña D.
dc.contributor.authorAbalos P.
dc.date.accessioned2020-09-02T22:26:55Z
dc.date.available2020-09-02T22:26:55Z
dc.date.issued2015
dc.identifier10.3389/fmicb.2015.00464
dc.identifier.citation6, MAY, -
dc.identifier.issn1664302X
dc.identifier.urihttps://hdl.handle.net/20.500.12728/5983
dc.descriptionSalmonella enterica serotype Enteritidis is a worldwide zoonotic agent that has been recognized as a very important food-borne bacterial pathogen, mainly associated with consumption of poultry products. The aim of this work was to determine genotypic and phenotypic evidence of S. Enteritidis transmission among seabirds, poultry and humans in Chile. Genotyping was performed using PCR-based virulotyping, pulse-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Pathogenicity-associated phenotypes were determined with survival to free radicals, acidic pH, starvation, antimicrobial resistance, and survival within human dendritic cells. As result of PCR and PFGE assays, some isolates from the three hosts showed identical genotypic patterns, and through MLST it was determined that all of them belong to sequence type 11. Phenotypic assays show diversity of bacterial responses among isolates. When results were analyzed according to bacterial host, statistical differences were identified in starvation and dendritic cells survival assays. In addition, isolates from seabirds showed the highest rates of resistance to gentamycin, tetracycline, and ampicillin. Overall, the very close genetic and phenotypic traits shown by isolates from humans, poultry, and seabirds suggest the inter-species transmission of S. Enteritidis bacteria between hosts, likely through anthropogenic environmental contamination that determines infection of seabirds with bacteria that are potentially pathogenic for other susceptible organism, including humans. © 2015 Retamal, Fresno, Dougnac, Gutierrez, Gornall, Vidal, Vernal, Pujol, Barreto, González-Acuña and Abalos.
dc.language.isoen
dc.publisherFrontiers Media S.A.
dc.subjectChile
dc.subjectEnteritidis
dc.subjectHumans
dc.subjectPoultry
dc.subjectSalmonella enterica
dc.subjectSeabirds
dc.subjectamoxicillin plus clavulanic acid
dc.subjectampicillin
dc.subjectcefotaxime
dc.subjectcefradine
dc.subjectceftiofur
dc.subjectciprofloxacin
dc.subjectcotrimoxazole
dc.subjectenrofloxacin
dc.subjectfree radical
dc.subjectgentamicin
dc.subjecttetracycline
dc.subjectantibiotic resistance
dc.subjectantibiotic sensitivity
dc.subjectArticle
dc.subjectChile
dc.subjectcolony forming unit
dc.subjectcontrolled study
dc.subjectdendritic cell
dc.subjectgenotype phenotype correlation
dc.subjectgenotyping technique
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmultilocus sequence typing
dc.subjectnonhuman
dc.subjectpolymerase chain reaction
dc.subjectpoultry
dc.subjectpulsed field gel electrophoresis
dc.subjectSalmonella enterica serovar Enteritidis
dc.subjectseabird
dc.subjectstarvation
dc.subjectAnimalia
dc.subjectBacteria (microorganisms)
dc.subjectSalmonella enterica
dc.subjectSalmonella enteritidis
dc.titleGenetic and phenotypic evidence of the Salmonella enterica serotype Enteritidis human-animal interface in Chile
dc.typeArticle


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