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Conservation of small regulatory RNAs in Vibrio parahaemolyticus: Possible role of RNA-OUT encoded by the pathogenicity island (VPaI-7) of pandemic strains

dc.contributor.authorPlaza N.
dc.contributor.authorPérez-Reytor D.
dc.contributor.authorRamírez-Araya S.
dc.contributor.authorPavón A.
dc.contributor.authorCorsini G.
dc.contributor.authorLoyola D.E.
dc.contributor.authorJaña V.
dc.contributor.authorPavéz L.
dc.contributor.authorNavarrete P.
dc.contributor.authorBastías R.
dc.contributor.authorCastillo D.
dc.contributor.authorGarcía K.
dc.date.accessioned2020-09-02T22:25:52Z
dc.date.available2020-09-02T22:25:52Z
dc.date.issued2019
dc.identifier10.3390/ijms20112827
dc.identifier.citation20, 11, -
dc.identifier.issn16616596
dc.identifier.urihttps://hdl.handle.net/20.500.12728/5817
dc.descriptionSmall regulatory RNAs (sRNAs) are molecules that play an important role in the regulation of gene expression. sRNAs in bacteria can affect important processes, such as metabolism and virulence. Previous studies showed a significant role of sRNAs in the Vibrio species, but knowledge about Vibrio parahaemolyticus is limited. Here, we examined the conservation of sRNAs between V. parahaemolyticus and other human Vibrio species, in addition to investigating the conservation between V. parahaemolyticus strains differing in pandemic origin. Our results showed that only 7% of sRNAs were conserved between V. parahaemolyticus and other species, but 88% of sRNAs were highly conserved within species. Nonetheless, two sRNAs coding to RNA-OUT, a component of the Tn10/IS10 system, were exclusively present in pandemic strains. Subsequent analysis showed that both RNA-OUT were located in pathogenicity island-7 and would interact with transposase VPA1379, according to the model of pairing of IS10-encoded antisense RNAs. According to the location of RNA-OUT/VPA1379, we also investigated if they were expressed during infection. We observed that the transcriptional level of VPA1379 was significantly increased, while RNA-OUT was decreased at three hours post-infection. We suggest that IS10 transcription increases in pandemic strains during infection, probably to favor IS10 transposition and improve their fitness when they are facing adverse conditions. © 2019 by the authors.
dc.language.isoen
dc.publisherMDPI AG
dc.subjectAntisense
dc.subjectIS10
dc.subjectRNA-OUT
dc.subjectSRNA
dc.subjectSvpa1401.1
dc.subjectSvpa1453.1
dc.subjectTransposase
dc.subjectVibrio parahaemolyticus
dc.subjectVPA1379
dc.subjectbacterial RNA
dc.subjectcomplementary RNA
dc.subjecttransfer RNA
dc.subjecttransposase
dc.subjectuntranslated RNA
dc.subjectArticle
dc.subjectbacterial metabolism
dc.subjectbacterial strain
dc.subjectbacterial virulence
dc.subjectgene expression regulation
dc.subjectgenetic conservation
dc.subjectgenetic transcription
dc.subjectnonhuman
dc.subjectpandemic
dc.subjectpathogenicity island
dc.subjectregulatory mechanism
dc.subjectVibrio
dc.subjectVibrio cholerae
dc.subjectVibrio parahaemolyticus
dc.subjectCaco-2 cell line
dc.subjectconserved sequence
dc.subjectgenetics
dc.subjectgenomic island
dc.subjecthuman
dc.subjectmetabolism
dc.subjectpathogenicity
dc.subjectVibrio parahaemolyticus
dc.subjectCaco-2 Cells
dc.subjectConserved Sequence
dc.subjectGenomic Islands
dc.subjectHumans
dc.subjectRNA, Untranslated
dc.subjectTransposases
dc.subjectVibrio parahaemolyticus
dc.titleConservation of small regulatory RNAs in Vibrio parahaemolyticus: Possible role of RNA-OUT encoded by the pathogenicity island (VPaI-7) of pandemic strains
dc.typeArticle


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