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Extremely high UV-C radiation resistant microorganisms from desert environments with different manganese concentrations

dc.contributor.authorPaulino-Lima I.G.
dc.contributor.authorFujishima K.
dc.contributor.authorNavarrete J.U.
dc.contributor.authorGalante D.
dc.contributor.authorRodrigues F.
dc.contributor.authorAzua-Bustos A.
dc.contributor.authorRothschild L.J.
dc.date.accessioned2020-09-02T22:25:25Z
dc.date.available2020-09-02T22:25:25Z
dc.date.issued2016
dc.identifier10.1016/j.jphotobiol.2016.08.017
dc.identifier.citation163, , 327-336
dc.identifier.issn10111344
dc.identifier.urihttps://hdl.handle.net/20.500.12728/5769
dc.descriptionDesiccation resistance and a high intracellular Mn/Fe ratio contribute to ionizing radiation resistance of Deinococcus radiodurans. We hypothesized that this was a general phenomenon and thus developed a strategy to search for highly radiation-resistant organisms based on their natural environment. While desiccation is a typical feature of deserts, the correlation between radiation resistance and the intracellular Mn/Fe ratio of indigenous microorganisms or the Mn/Fe ratio of the environment, has not yet been described. UV-C radiation is highly damaging to biomolecules including DNA. It was used in this study as a selective tool because of its relevance to early life on earth, high altitude aerobiology and the search for life beyond Earth. Surface soil samples were collected from the Sonoran Desert, Arizona (USA), from the Atacama Desert in Chile and from a manganese mine in northern Argentina. Microbial isolates were selected after exposure to UV-C irradiation and growth. The isolates comprised 28 genera grouped within six phyla, which we ranked according to their resistance to UV-C irradiation. Survival curves were performed for the most resistant isolates and correlated with their intracellular Mn/Fe ratio, which was determined by ICP-MS. Five percent of the isolates were highly resistant, including one more resistant than D. radiodurans, a bacterium generally considered the most radiation-resistant organism, thus used as a model for radiation resistance studies. No correlation was observed between the occurrence of resistant microorganisms and the Mn/Fe ratio in the soil samples. However, all resistant isolates showed an intracellular Mn/Fe ratio much higher than the sensitive isolates. Our findings could represent a new front in efforts to harness mechanisms of UV-C radiation resistance from extreme environments. © 2016 Elsevier B.V.
dc.language.isoen
dc.publisherElsevier B.V.
dc.subjectDesiccation
dc.subjectExtremophile
dc.subjectManganese
dc.subjectMicrobial diversity
dc.subjectUltraviolet radiation resistance
dc.subjectiron
dc.subjectmanganese
dc.subjectiron
dc.subjectmanganese
dc.subjectArgentina
dc.subjectArticle
dc.subjectbacterium isolate
dc.subjectChile
dc.subjectDeinococcus radiodurans
dc.subjectdesert
dc.subjectdesiccation
dc.subjectmass spectrometry
dc.subjectmicroorganism detection
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpriority journal
dc.subjectradiation exposure
dc.subjectradiosensitivity
dc.subjectsurface soil
dc.subjectsurvival rate
dc.subjectultraviolet C radiation
dc.subjectUnited States
dc.subjectdesert climate
dc.subjectdrug effects
dc.subjectextracellular space
dc.subjectintracellular space
dc.subjectmetabolism
dc.subjectmicrobiology
dc.subjectphylogeny
dc.subjectradiation response
dc.subjectradiation tolerance
dc.subjectultraviolet radiation
dc.subjectDesert Climate
dc.subjectExtracellular Space
dc.subjectIntracellular Space
dc.subjectIron
dc.subjectManganese
dc.subjectPhylogeny
dc.subjectRadiation Tolerance
dc.subjectSoil Microbiology
dc.subjectUltraviolet Rays
dc.titleExtremely high UV-C radiation resistant microorganisms from desert environments with different manganese concentrations
dc.typeArticle


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