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Indirect immunofluorescence technique versus polymerase chain reaction for the diagnosis of respiratory viruses in children admitted to a hospital in the Metropolitan Region [Inmunofluorescencia indirecta versus reacción de polimerasa en cadena para el diagnóstico de virus respiratorios en niños ingresados en un hospital de la Región Metropolitana]

dc.contributor.authorPablo Corvalán L.
dc.contributor.authorGuisselle Arias B.
dc.contributor.authorPaola Morales S.
dc.contributor.authorRaquel González M.
dc.contributor.authorJaime Inostroza S.
dc.contributor.authorLoreto Fuenzalida I.
dc.date.accessioned2020-09-02T22:25:14Z
dc.date.available2020-09-02T22:25:14Z
dc.date.issued2019
dc.identifier10.4067/S0716-10182019000100026
dc.identifier.citation36, 1, 26-31
dc.identifier.issn07161018
dc.identifier.urihttps://hdl.handle.net/20.500.12728/5705
dc.descriptionBackground: Early viral detection in acute respiratory infections (ARI) is essential to establish appropriate therapy and prevent nosocomial transmission. Objective: To compare the efficacy of indirect immunofluorescence technique (IIF) with the polymerase chain reaction (PCR) to identify respiratory viruses in children hospitalized for ARI. Methods: 47 nasopharyngeal aspirates of children = 2 years with ARI were included. IFI included respiratory syncytial virus (RSV), adenovirus, influenza A and B and parainfluenza. PCR also included the detection of metapneumovirus, enterovirus/rhinovirus, bocavirus and coronavirus. Sensitivity, specificity, positive and negative predictive value (VPP/NPV) and kappa correlation for RSV were estimated by IIF compared to PCR. Results: The IIF detected only RSV (29; 61.7%). PCR detected several viruses, including RSV in 26 cases (55.3%), followed by bocavirus (29.8%), rhinovirus/enterovirus (21.3%), adenovirus (14.9%) and parainfluenza (4,3%) among others, with 35.5% of coinfection. The IIF presented sensitivity: 85.7%, specificity: 73.6%, PPV: 82.7%, NPV: 77.7% and kappa: 0.5990 (95% CI, 0.3636-0.8346) for RSV. Conclusion: The IIF presents good sensitivity, but moderate specificity for RSV. However, IIF fails to detect other respiratory viruses. The introduction of PCR would improve the etiological diagnosis of ARI of viral origin. © 2019, Sociedad Chilena de Infectologia. All rights reserved.
dc.language.isoes
dc.publisherSociedad Chilena de Infectologia
dc.subjectAcute respiratory infection
dc.subjectIndirect immunofluorescence
dc.subjectPolymerase chain reaction
dc.subjectRespiratory viruses
dc.subjectDNA virus
dc.subjectadolescent
dc.subjectchild
dc.subjectChile
dc.subjectcomparative study
dc.subjectcross-sectional study
dc.subjectfemale
dc.subjecthuman
dc.subjectindirect fluorescent antibody technique
dc.subjectinfant
dc.subjectisolation and purification
dc.subjectmale
dc.subjectnasopharynx
dc.subjectpolymerase chain reaction
dc.subjectpreschool child
dc.subjectprocedures
dc.subjectprospective study
dc.subjectreproducibility
dc.subjectrespiratory tract infection
dc.subjectRNA virus
dc.subjectsensitivity and specificity
dc.subjectvirology
dc.subjectvirus
dc.subjectAdolescent
dc.subjectChild
dc.subjectChild, Preschool
dc.subjectChile
dc.subjectCross-Sectional Studies
dc.subjectDNA Viruses
dc.subjectFemale
dc.subjectFluorescent Antibody Technique, Indirect
dc.subjectHumans
dc.subjectInfant
dc.subjectMale
dc.subjectNasopharynx
dc.subjectPolymerase Chain Reaction
dc.subjectProspective Studies
dc.subjectReproducibility of Results
dc.subjectRespiratory Tract Infections
dc.subjectRNA Viruses
dc.subjectSensitivity and Specificity
dc.subjectViruses
dc.titleIndirect immunofluorescence technique versus polymerase chain reaction for the diagnosis of respiratory viruses in children admitted to a hospital in the Metropolitan Region [Inmunofluorescencia indirecta versus reacción de polimerasa en cadena para el diagnóstico de virus respiratorios en niños ingresados en un hospital de la Región Metropolitana]
dc.typeArticle


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