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Enhancement of cytotoxic activity by encapsulation in pluronic polymer micelles: Leptocarpha rivularis extracts against human cancer cell lines

dc.contributor.authorOlea A.F.
dc.contributor.authorVillena J.
dc.contributor.authorMoller A.
dc.contributor.authorMartínez R.
dc.contributor.authorCarrasco H.
dc.date.accessioned2020-09-02T22:24:45Z
dc.date.available2020-09-02T22:24:45Z
dc.date.issued2019
dc.identifier10.4067/S0717-97072019000204437
dc.identifier.citation64, 2, 4437-4440
dc.identifier.issn07179324
dc.identifier.urihttps://hdl.handle.net/20.500.12728/5636
dc.descriptionLeptocarpha rivularis is an autochthonous plant of the southern zone of Chile known as “Palo negro” that has been traditionally used by Mapuche people as anti-inflammatory and reliever of abdominal pain. More recently, aqueous infusion of this plant is being used in the treatment of cancer. The main phytochemical components of L. rivularis have been identified as a sesquiterpene lactones (SQL) being leptocarpine (LTC) the major component, which exhibits pro-apoptotic action against a variety of cancer cells lines. In this study we intend to compare the in vitro cytotoxicity against cancer cell lines of two different polarity extracts and LTC. To overcome the poor water-solubility of extracts they have been encapsulated in polymer micelles and its effect on activity has been assessed. The results indicate that both extracts reduce cell viability of all cancer cell lines. The IC50 values obtDLQHG IRU HWK\O DFHWDWH DUH LQ WKH UDQJH RI 11í16 μJ/P/, DQG this activity is explained in terms of a synergic effect generated by SQL different to LTC. The IC50 values of extracts incorporated into polymer micelles formed by Pluronic F127 are 200 times lower than those measured for ethyl acetate extract applied in homogeneous solution. Finally, by assessing changes in cell and nuclear morphology it is suggested that the observed cytotoxicity of free and entrapped extracts is produced by apoptotic process. In conclusion, these results suggest that plant extracts may show an increased activity as result of synergic effect of minority components, and an important enhancement of cytotoxicity is induced by entrapment into polymer micelles. © 2019 Sociedad Chilena de Quimica. All rights reserved.
dc.language.isoen
dc.publisherSociedad Chilena de Quimica
dc.subjectApoptosis
dc.subjectCytotoxicity
dc.subjectLeptocarpine
dc.subjectPlant extracts
dc.subjectPolymer micelles
dc.subjectSesquiterpene lactones
dc.titleEnhancement of cytotoxic activity by encapsulation in pluronic polymer micelles: Leptocarpha rivularis extracts against human cancer cell lines
dc.typeArticle


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