Mostrar el registro sencillo del ítem

Effectiveness of sampling methods employed for Acanthamoeba keratitis diagnosis by culture

dc.contributor.authorMuiño L.
dc.contributor.authorRodrigo D.
dc.contributor.authorVillegas R.
dc.contributor.authorRomero P.
dc.contributor.authorPeredo D.E.
dc.contributor.authorVargas R.A.
dc.contributor.authorLiempi D.
dc.contributor.authorOsuna A.
dc.contributor.authorJercic M.I.
dc.date.accessioned2020-09-02T22:23:44Z
dc.date.available2020-09-02T22:23:44Z
dc.date.issued2019
dc.identifier10.1007/s10792-018-0958-3
dc.identifier.citation39, 7, 1451-1458
dc.identifier.issn01655701
dc.identifier.urihttps://hdl.handle.net/20.500.12728/5465
dc.descriptionPurpose: This retrospective, observational study was designed to evaluate the effectiveness of the sampling methods commonly used for the collection of corneal scrapes for the diagnosis of Acanthamoeba keratitis (AK) by culture, in terms of their ability to provide a positive result. Methods: A total of 553 samples from 380 patients with suspected AK received at the Parasitology Section of the Public Health Institute of Chile, between January 2005 and December 2015, were evaluated. A logistic regression model was used to determine the correlation between the culture outcome (positive or negative) and the method for sample collection. The year of sample collection was also included in the analysis as a confounding variable. Results: Three hundred and sixty-five samples (27%) from 122 patients (32.1%) were positive by culture. The distribution of sample types was as follows: 142 corneal scrapes collected using a modified bezel needle (a novel method developed by a team of Chilean corneologists), 176 corneal scrapes obtained using a scalpel, 50 corneal biopsies, 30 corneal swabs, and 155 non-biological materials including contact lens and its paraphernalia. Biopsy provided the highest likelihood ratio for a positive result by culture (1.89), followed by non-biological materials (1.10) and corneal scrapes obtained using a modified needle (1.00). The lowest likelihood ratio was estimated for corneal scrapes obtained using a scalpel (0.88) and cotton swabs (0.78). Conclusion: Apart from biopsy, optimum corneal samples for the improved diagnosis of AK can be obtained using a modified bezel needle instead of a scalpel, while cotton swabs are not recommended. © 2018, Springer Nature B.V.
dc.language.isoen
dc.publisherSpringer Netherlands
dc.subjectAcanthamoeba
dc.subjectContact lens
dc.subjectEye pathogens
dc.subjectKeratitis diagnosis
dc.subjectAcanthamoeba keratitis
dc.subjectArticle
dc.subjectChile
dc.subjectclinical effectiveness
dc.subjectcorneal biopsy
dc.subjectcorrelational study
dc.subjectfemale
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectobservational study
dc.subjectparasite examination
dc.subjectretrospective study
dc.subjectsampling
dc.subjectvalidation process
dc.subjectAcanthamoeba
dc.subjectAcanthamoeba keratitis
dc.subjectclinical trial
dc.subjectconfocal microscopy
dc.subjectcornea
dc.subjectgenetics
dc.subjectisolation and purification
dc.subjectmulticenter study
dc.subjectparasitic eye infection
dc.subjectparasitology
dc.subjectpathology
dc.subjectpolymerase chain reaction
dc.subjectprocedures
dc.subjectprotozoal DNA
dc.subjectAcanthamoeba
dc.subjectAcanthamoeba Keratitis
dc.subjectCornea
dc.subjectDNA, Protozoan
dc.subjectEye Infections, Parasitic
dc.subjectFemale
dc.subjectHumans
dc.subjectMale
dc.subjectMicroscopy, Confocal
dc.subjectPolymerase Chain Reaction
dc.subjectRetrospective Studies
dc.titleEffectiveness of sampling methods employed for Acanthamoeba keratitis diagnosis by culture
dc.typeArticle


Ficheros en el ítem

Thumbnail
Nombre:
item_2-s2.0-85048698895.pdf
Tamaño:
3.628Kb
Formato:
PDF
Ver/

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem