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Lipase From Rhizomucor miehei Immobilized on Magnetic Nanoparticles: Performance in Fatty Acid Ethyl Ester (FAEE) Optimized Production by the Taguchi Method

dc.contributor.authorMoreira K.D.S.
dc.contributor.authorde Oliveira A.L.B.
dc.contributor.authorJúnior L.S.D.M.
dc.contributor.authorMonteiro R.R.C.
dc.contributor.authorda Rocha T.N.
dc.contributor.authorMenezes F.L.
dc.contributor.authorFechine L.M.U.D.
dc.contributor.authorDenardin J.C.
dc.contributor.authorMichea S.
dc.contributor.authorFreire R.M.
dc.contributor.authorFechine P.B.A.
dc.contributor.authorSouza M.C.M.
dc.contributor.authordos Santos J.C.S.
dc.date.accessioned2020-09-02T22:23:40Z
dc.date.available2020-09-02T22:23:40Z
dc.date.issued2020
dc.identifier10.3389/fbioe.2020.00693
dc.identifier.citation8, , -
dc.identifier.issn22964185
dc.identifier.urihttps://hdl.handle.net/20.500.12728/5443
dc.descriptionIn this communication, it was evaluated the production of fatty acid ethyl ester (FAAE) from the free fatty acids of babassu oil catalyzed by lipase from Rhizomucor miehei (RML) immobilized on magnetic nanoparticles (MNP) coated with 3-aminopropyltriethoxysilane (APTES), Fe3O4@APTES-RML or RML-MNP for short. MNPs were prepared by co-precipitation coated with 3-aminopropyltriethoxysilane and used as a support to immobilize RML (immobilization yield: 94.7 ± 1.0%; biocatalyst activity: 341.3 ± 1.2 Up–NPB/g), which were also activated with glutaraldehyde and then used to immobilize RML (immobilization yield: 91.9 ± 0.2%; biocatalyst activity: 199.6 ± 3.5 Up–NPB/g). RML-MNP was characterized by X-Ray Powder Diffraction (XRPD), Fourier Transform-Infrared (FTIR) spectroscopy and Scanning Electron Microscope (SEM), proving the incorporation and immobilization of RML on the APTES matrix. In addition, the immobilized biocatalyst presented at 60°C a half-life 16–19 times greater than that of the soluble lipase in the pH range 5–10. RML and RML-MNP showed higher activity at pH 7; the immobilized enzyme was more active than the free enzyme in the pH range (5–10) analyzed. For the production of fatty acid ethyl ester, under optimal conditions [40°C, 6 h, 1:1 (FFAs/alcohol)] determined by the Taguchi method, it was possible to obtain conversion of 81.7 ± 0.7% using 5% of RML-MNP. © Copyright © 2020 Moreira, de Oliveira, Júnior, Monteiro, da Rocha, Menezes, Fechine, Denardin, Michea, Freire, Fechine, Souza and dos Santos.
dc.language.isoen
dc.publisherFrontiers Media S.A.
dc.subjectAPTES
dc.subjectfatty acid ethyl ester
dc.subjectimmobilized
dc.subjectlipase from Rhizomucor miehei
dc.subjectmagnetic nanoparticles
dc.subjectTaguchi
dc.subjectEnzymes
dc.subjectEsters
dc.subjectFourier transform infrared spectroscopy
dc.subjectIron oxides
dc.subjectIsomers
dc.subjectMagnetic nanoparticles
dc.subjectMagnetite
dc.subjectpH
dc.subjectScanning electron microscopy
dc.subjectTaguchi methods
dc.subjectX ray powder diffraction
dc.subject3-aminopropyltriethoxysilane
dc.subjectFatty acid ethyl ester
dc.subjectGlutaraldehydes
dc.subjectImmobilized biocatalysts
dc.subjectImmobilized enzyme
dc.subjectOptimal conditions
dc.subjectOptimized production
dc.subjectRhizomucor miehei
dc.subjectFatty acids
dc.titleLipase From Rhizomucor miehei Immobilized on Magnetic Nanoparticles: Performance in Fatty Acid Ethyl Ester (FAEE) Optimized Production by the Taguchi Method
dc.typeArticle


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