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Immunostimulatory activity of low-molecular-weight hyaluronan on dendritic cells stimulated with Aggregatibacter actinomycetemcomitans or Porphyromonas gingivalis

dc.contributor.authorMonasterio G.
dc.contributor.authorGuevara J.
dc.contributor.authorIbarra J.P.
dc.contributor.authorCastillo F.
dc.contributor.authorDíaz-Zúñiga J.
dc.contributor.authorAlvarez C.
dc.contributor.authorCafferata E.A.
dc.contributor.authorVernal R.
dc.date.accessioned2020-09-02T22:23:31Z
dc.date.available2020-09-02T22:23:31Z
dc.date.issued2019
dc.identifier10.1007/s00784-018-2641-5
dc.identifier.citation23, 4, 1887-1894
dc.identifier.issn14326981
dc.identifier.urihttps://hdl.handle.net/20.500.12728/5378
dc.descriptionObjectives: Periodontitis is a chronic inflammatory disease characterized by tooth-supporting tissue destruction, which is elicited by the host’s immune response triggered against periodonto-pathogen bacteria. During periodontal tissue destruction, extracellular matrix components are metabolized and fragmented. Some extracellular matrix component-derived fragments, such as low-molecular-weight hyaluronan (LMW-HA), have potent immunogenic potential, playing a role as damage-associated molecular patterns (DAMPs) during activation of immune cells. Dendritic cells (DCs) play a central role in the host’s immune response displayed during periodontitis; thus, this study aimed to analyze whether LMW-HA has an immunostimulatory activity on DCs when stimulated with periodonto-pathogen bacteria. Materials and methods: LMW-HA-treated and non-treated DCs were stimulated with Aggregatibacter actinomycetemcomitans or Porphyromonas gingivalis and the mRNA expression for cytokines tumor necrosis factor-α (TNF-alpha), interleukin-1β (IL-1B), interleukin-6 (IL-6), and interleukin-23 (IL-23A) was quantified by RT-qPCR. In addition, transcription factors interferon regulatory factor 4 (IRF4), interferon regulatory factor 8 (IRF8), neurogenic locus notch homolog protein 2 (NOTCH2), and basic leucine zipper ATF-like transcription factor 3 (BATF3), involved in DC activation, were analyzed. Results: Higher expression levels of TNF-alpha, IL-1B, IL-6, and IL-23A were detected in LMW-HA-treated DCs after bacterial infection, as compared with non-treated DCs. When LMW-HA-treated DCs were infected with A. actinomycetemcomitans, higher levels of IRF4, NOTCH2, and BATF3 were detected compared with non-treated cells; whereas against P. gingivalis infection, increased levels of IRF4 and NOTCH2 were detected. Conclusion: LMW-HA plays an immunostimulatory role on the immune response triggered by DCs during infection with A. actinomycetemcomitans or P. gingivalis. Clinical relevance: Detection of extracellular matrix component-derived fragments produced during periodontal tissue destruction, such as LMW-HA, could explain at least partly unsuccessful periodontal treatment and the chronicity of the disease. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
dc.language.isoen
dc.publisherSpringer Verlag
dc.subjectAggregatibacter actinomycetemcomitans
dc.subjectCytokines
dc.subjectDendritic cells
dc.subjectHyaluronan
dc.subjectLMW-HA
dc.subjectPorphyromonas gingivalis
dc.subjectTranscription factors
dc.subjectcytokine
dc.subjecthyaluronic acid
dc.subjectimmunological adjuvant
dc.subjectAggregatibacter actinomycetemcomitans
dc.subjectcell culture
dc.subjectdendritic cell
dc.subjectdrug effect
dc.subjectextracellular matrix
dc.subjecthuman
dc.subjectimmunology
dc.subjectmicrobiology
dc.subjectmolecular weight
dc.subjectperiodontitis
dc.subjectPorphyromonas gingivalis
dc.subjectAdjuvants, Immunologic
dc.subjectAggregatibacter actinomycetemcomitans
dc.subjectCells, Cultured
dc.subjectCytokines
dc.subjectDendritic Cells
dc.subjectExtracellular Matrix
dc.subjectHumans
dc.subjectHyaluronic Acid
dc.subjectMolecular Weight
dc.subjectPeriodontitis
dc.subjectPorphyromonas gingivalis
dc.titleImmunostimulatory activity of low-molecular-weight hyaluronan on dendritic cells stimulated with Aggregatibacter actinomycetemcomitans or Porphyromonas gingivalis
dc.typeArticle


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