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Alveolar bone resorption and TH1/TH17-associated immune response triggered during Aggregatibacter actinomycetemcomitans-induced experimental periodontitis are serotype-dependent

dc.contributor.authorMonasterio G.
dc.contributor.authorCastillo F.
dc.contributor.authorIbarra J.P.
dc.contributor.authorGuevara J.
dc.contributor.authorRojas L.
dc.contributor.authorAlvarez C.
dc.contributor.authorFernández B.
dc.contributor.authorAgüero A.
dc.contributor.authorBetancur D.
dc.contributor.authorVernal R.
dc.date.accessioned2020-09-02T22:23:30Z
dc.date.available2020-09-02T22:23:30Z
dc.date.issued2018
dc.identifier10.1002/JPER.17-0563
dc.identifier.citation89, 10, 1249-1261
dc.identifier.issn00223492
dc.identifier.urihttps://hdl.handle.net/20.500.12728/5375
dc.descriptionBackground: Aggregatibacter actinomycetemcomitans expresses several virulence factors that may contribute to the pathogenesis of periodontitis. Based on the anti-genicity of the O-polysaccharide component of the lipopolysaccharide (LPS), different A. actinomycetemcomitans serotypes have been described. Among them, serotype b has demonstrated a stronger capacity to trigger Th1 and Th17-associated cytokine, CC-chemokine, and CC-chemokine receptor production on immune cells in vitro. With a murine model of experimental periodontitis, this investigation aimed to analyze the alveolar bone resorption and the pattern of immune response triggered by the different A. actinomycetemcomitans serotypes within periodontal lesions. Methods: For periodontal lesion induction, mice were orally infected with the different A. actinomycetemcomitans serotypes or their purified LPS. Alveolar bone resorption was analyzed using microcomputed tomography and scanning electron microscopy. Bacterial infection, receptor activator of nuclear factor-kappa B ligand (RANKL) and Th1 and Th17-associated cytokine, CC-chemokine, and CC-chemokine receptor levels were quantified by quantitative polymerase chain reaction (qPCR). T lymphocytes isolated from periodontal lesions were analyzed by flow cytometry. Results: In periodontal lesions, serotype b of A. actinomycetemcomitans induced higher alveolar bone resorption and expression of RANKL compared with the other serotypes. In addition, serotype b induced greater levels of Th1-and Th17-related cytokines, CC-chemokines, and CC-chemokine receptors than the others. Similarly, higher numbers of infiltrating Th1 and Th17 lymphocytes were detected in serotype b-induced periodontal lesions. Conclusions: These results demonstrate that periodontal lesions induced with different A. actinomycetemcomitans serotypes elicited distinct alveolar bone resorption and immune response. In particular, serotype b was more pathogenic than the others and induced stronger Th1 and Th17 patterns of immune responses during experimental periodontitis. © 2018 American Academy of Periodontology.
dc.language.isoen
dc.publisherWiley-Blackwell
dc.subjectAggregatibacter actinomycetemcomitans
dc.subjectBone resorption
dc.subjectChemokine receptors
dc.subjectChemokines
dc.subjectCytokines
dc.subjectRANKL
dc.subjectT lymphocytes
dc.subjectAggregatibacter actinomycetemcomitans
dc.subjectanimal
dc.subjectmicro-computed tomography
dc.subjectmouse
dc.subjectperiodontitis
dc.subjectserotype
dc.subjectTh17 cell
dc.subjectAggregatibacter actinomycetemcomitans
dc.subjectAnimals
dc.subjectMice
dc.subjectPeriodontitis
dc.subjectSerogroup
dc.subjectTh17 Cells
dc.subjectX-Ray Microtomography
dc.titleAlveolar bone resorption and TH1/TH17-associated immune response triggered during Aggregatibacter actinomycetemcomitans-induced experimental periodontitis are serotype-dependent
dc.typeArticle


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