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DT-Diaphorase Prevents Aminochrome-Induced Lysosome Dysfunction in SH-SY5Y Cells

dc.contributor.authorMeléndez C.
dc.contributor.authorMuñoz P.
dc.contributor.authorSegura-Aguilar J.
dc.date.accessioned2020-09-02T22:22:54Z
dc.date.available2020-09-02T22:22:54Z
dc.date.issued2019
dc.identifier10.1007/s12640-018-9953-8
dc.identifier.citation35, 1, 255-259
dc.identifier.issn10298428
dc.identifier.urihttps://hdl.handle.net/20.500.12728/5289
dc.descriptionAminochrome has been reported to induce lysosomal dysfunction by inhibiting the vacuolar H-type ATPase localized in lysosome membrane. DT-diaphorase has been proposed to prevent aminochrome neurotoxicity but it is unknown whether this enzyme prevents aminochrome-induced lysosomal dysfunction. In the present study, we tested the protective role of DT-diaphorase in lysosomal dysfunction by generating a cell line (SH-SY5YsiNQ7) with a stable expression of a siRNA against DT-diaphorase with only 10% expression of mRNA enzyme. The cells differentiated with retinoic acid and 12-o-tetradecanoylphorbol-13-acetate show a significant increase in the expression of tyrosine hydroxylase, vesicular monoamine transporter-2, and dopamine transporter. The incubation of SH-SY5YsiNQ7 cells with 10 μM aminochrome resulted in a significant decrease of lysosome pH determined by using acridine orange, while aminochrome has no effect on SH-SY5Y cells. These results support the proposed protective role of DT-diaphorase against aminochrome-induced lysosomal dysfunction. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
dc.language.isoen
dc.publisherSpringer New York LLC
dc.subjectAminochrome
dc.subjectDopamine
dc.subjectDT-Diaphorase
dc.subjectLysosomal dysfunction
dc.subjectNeuroprotection
dc.subjectNeurotoxicity
dc.subject4 o methylphorbol 13 acetate 12 myristate
dc.subjectacridine orange
dc.subjectaminochrome
dc.subjectdihydrolipoamide dehydrogenase
dc.subjectdopamine transporter
dc.subjectneurotoxin
dc.subjectretinoic acid
dc.subjecttyrosine 3 monooxygenase
dc.subjectunclassified drug
dc.subjectvesicular monoamine transporter 2
dc.subjectaminochrome 1
dc.subjectindole derivative
dc.subjectreduced nicotinamide adenine dinucleotide (phosphate) dehydrogenase (quinone)
dc.subjectArticle
dc.subjectcell damage
dc.subjectcell differentiation
dc.subjectcontrolled study
dc.subjectgene expression
dc.subjectimmunofluorescence
dc.subjectincubation time
dc.subjectlysosome
dc.subjectpH
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectreal time polymerase chain reaction
dc.subjectSH-SY5Y cell line
dc.subjectanimal
dc.subjectantagonists and inhibitors
dc.subjectdrug effect
dc.subjectenzymology
dc.subjectgenetics
dc.subjectlysosome
dc.subjectmetabolism
dc.subjectmouse
dc.subjectneuroprotection
dc.subjectphysiology
dc.subjecttumor cell line
dc.subjectAnimals
dc.subjectCell Line, Tumor
dc.subjectHydrogen-Ion Concentration
dc.subjectIndolequinones
dc.subjectLysosomes
dc.subjectMice
dc.subjectNAD(P)H Dehydrogenase (Quinone)
dc.subjectNeuroprotection
dc.titleDT-Diaphorase Prevents Aminochrome-Induced Lysosome Dysfunction in SH-SY5Y Cells
dc.typeArticle


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