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Hepatic Nrf2 expression is altered by quercetin supplementation in X.irradiated rats

dc.contributor.authorMarina R.
dc.contributor.authorGonzalez P.
dc.contributor.authorCarmen Ferreras M.
dc.contributor.authorCostilla S.
dc.contributor.authorBarrio J.-P.
dc.date.accessioned2020-09-02T22:22:22Z
dc.date.available2020-09-02T22:22:22Z
dc.date.issued2015
dc.identifier10.3892/mmr.2014.2741
dc.identifier.citation11, 1, 539-546
dc.identifier.issn17912997
dc.identifier.urihttps://hdl.handle.net/20.500.12728/5207
dc.descriptionWhole-body irradiation has been associated with liver function alterations. Ionizing radiation exposure increases oxidative stress and antioxidants can activate transcription of antioxidant target genes. In the present study, modifications of the liver antioxidant system were evaluated at 7 and 30 days following sub-lethal whole-body X.irradiation in male Wistar rats, which were intragastrically supplemented with quercetin or control solvent for 4 days prior to and 6 days following irradiation. Animal groups were as follows: CS, control, solvent.supplemented; CQ, control, quercetin-supplemented; RS, irradiated, solvent.supplemented; and RQ, irradiated, quercetin-supplemented. After 7 days, liver tissue from RS animals demonstrated marked hydropic panlobular degeneration with Mallory bodies in ballooning hepatocytes. These changes were mostly reversed in RQ rats. Lipid peroxidation in addition to copper/zinc superoxide dismutase (Cu/Zn.SOD), nuclear factor (erythroid.derived 2).like 2 (Nrf2) and Kelch-like ECH-associated protein 1 (Keap1) protein expression levels were all increased by X-irradiation, but significantly decreased by quercetin supplementation. Catalase (CAT) and NAD(P)H: quinone oxidoreductase 1 (NQO1) expression levels remained high in irradiated rats regardless of quercetin supplementation. After 30 days, the liver from RS animals had small portal infiltrates and diffuse cytoplasmic vacuolization, with reduced lipid peroxidation and reduced expression levels of CAT, NQO1, Nrf2 and Keap1, but consistently elevated Cu/Zn-SOD expression. RQ animals indicated reduced expression levels of Nrf2 and Keap1 30 days after irradiation. The present study demonstrated a quercetin-induced reduction of the oxidative stress-associated increase in Nrf2 expression that may be useful for preventing cancer cell survival in response to ionizing radiation exposure.
dc.language.isoen
dc.publisherSpandidos Publications
dc.subjectAntioxidants
dc.subjectLiver
dc.subjectNrf2
dc.subjectOxidative Stress
dc.subjectQuercetin
dc.subjectRadiation
dc.subjectRat
dc.subjectcatalase
dc.subjectcopper zinc superoxide dismutase
dc.subjectkelch like ECH associated protein 1
dc.subjectquercetin
dc.subjectreduced nicotinamide adenine dinucleotide (phosphate) dehydrogenase (quinone)
dc.subjectsolvent
dc.subjecttranscription factor Nrf2
dc.subjectantioxidant
dc.subjectbiological marker
dc.subjectKEAP1 protein, rat
dc.subjectquercetin
dc.subjectsignal peptide
dc.subjecttranscription factor Nrf2
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectantioxidant activity
dc.subjectArticle
dc.subjectcell degeneration
dc.subjectcell structure
dc.subjectcell vacuole
dc.subjectcontrolled study
dc.subjectdiet supplementation
dc.subjectionizing radiation
dc.subjectlipid peroxidation
dc.subjectliver
dc.subjectliver cell damage
dc.subjectliver function
dc.subjectmale
dc.subjectMallory body
dc.subjectnonhuman
dc.subjectoxidative stress
dc.subjectprotein expression
dc.subjectradiation exposure
dc.subjectrat
dc.subjectwhole body radiation
dc.subjectX irradiation
dc.subjectanimal
dc.subjectdrug effects
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectpathology
dc.subjectradiation response
dc.subjectAnimalia
dc.subjectRattus
dc.subjectRattus norvegicus
dc.subjectAnimals
dc.subjectAntioxidants
dc.subjectBiological Markers
dc.subjectGene Expression Regulation
dc.subjectIntracellular Signaling Peptides and Proteins
dc.subjectLipid Peroxidation
dc.subjectLiver
dc.subjectMale
dc.subjectNF-E2-Related Factor 2
dc.subjectOxidative Stress
dc.subjectQuercetin
dc.subjectRats
dc.titleHepatic Nrf2 expression is altered by quercetin supplementation in X.irradiated rats
dc.typeArticle


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