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Diversification of vibrio anguillarum driven by the bacteriophage CHOED

dc.contributor.authorLeón M.
dc.contributor.authorKokkari C.
dc.contributor.authorGarcía K.
dc.contributor.authorCastillo D.
dc.contributor.authorKatharios P.
dc.contributor.authorBastías R.
dc.date.accessioned2020-09-02T22:21:44Z
dc.date.available2020-09-02T22:21:44Z
dc.date.issued2019
dc.identifier10.3389/fmicb.2019.01396
dc.identifier.citation10, JUN, -
dc.identifier.issn1664302X
dc.identifier.urihttps://hdl.handle.net/20.500.12728/5075
dc.descriptionBacteriophages are an important factor in bacterial evolution. Some reports suggest that lytic bacteriophages can select for resistant mutant strains with reduced virulence. The present study explores the role of the CHOED bacteriophage in the diversification and virulence of its host Vibrio anguillarum. Nine phage-resistant strains were analyzed for their phenotype and different virulence factors, showing alterations in their fitness, motility, biofilm formation, lipopolysaccharide profiles and/or protease activity. Seven of the nine phage-resistant strains showed virulence reduction in a Sparus aurata larvae model. However, this is not generalized since two of the resistant strains show equal virulence compared with the parental strain. The genomic analysis of representative resistant strains displayed that the majority of the mutations are specific for each isolate, affecting genes related to lipopolysaccharide biosynthesis, quorum sensing, motility, toxin and membrane transport. The observed mutations were coherent with the phenotypic and virulence differences observed. These results suggest that the CHOED phage acts as a selective pressure on V. anguillarum, allowing proliferation of resistant strains with different genotypes, phenotypes and degrees of virulence, contributing to bacterial diversification. © 2007 - 2019 Frontiers Media S.A. All Rights Reserved.
dc.language.isoen
dc.publisherFrontiers Media S.A.
dc.subjectBacterial evolution
dc.subjectBacteriophage
dc.subjectBacteriophage resistance
dc.subjectDiversification
dc.subjectVibrio anguillarum
dc.subjectVirulence
dc.subjectbacterial DNA
dc.subjectbacterial toxin
dc.subjectlipopolysaccharide
dc.subjectproteinase
dc.subjectsiderophore
dc.subjectvirulence factor
dc.subjectArticle
dc.subjectbacterial growth
dc.subjectbacterial strain
dc.subjectbacterial virulence
dc.subjectbacteriophage
dc.subjectbacterium identification
dc.subjectbacterium isolation
dc.subjectcell motility
dc.subjectcell proliferation
dc.subjectDNA extraction
dc.subjectDNA sequence
dc.subjectelectron microscopy
dc.subjectfitness
dc.subjectgenetic identification
dc.subjectgenotype
dc.subjectgrowth curve
dc.subjecthemolysis
dc.subjectmicrobial diversity
dc.subjectnonhuman
dc.subjectphenotype
dc.subjectquorum sensing
dc.subjectSparus aurata
dc.subjectVibrio anguillarum
dc.titleDiversification of vibrio anguillarum driven by the bacteriophage CHOED
dc.typeArticle


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