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Amino Acid-Functionalized Polyelectrolyte Films as Bioactive Surfaces for Cell Adhesion

dc.contributor.authorLeal M.S.
dc.contributor.authorBriones X.
dc.contributor.authorVillalobos V.
dc.contributor.authorQueneau Y.
dc.contributor.authorLeiva A.
dc.contributor.authorRíos H.E.
dc.contributor.authorPavez J.
dc.contributor.authorSilva C.P.
dc.contributor.authorCarrasco C.
dc.contributor.authorNeira-Carrillo A.
dc.contributor.authorRoth A.D.
dc.contributor.authorTamayo L.
dc.contributor.authorUrzúa M.D.
dc.date.accessioned2020-09-02T22:21:18Z
dc.date.available2020-09-02T22:21:18Z
dc.date.issued2019
dc.identifier10.1021/acsami.9b02503
dc.identifier.citation11, 22, 19751-19762
dc.identifier.issn19448244
dc.identifier.urihttps://hdl.handle.net/20.500.12728/5064
dc.descriptionSurfaces were prepared with polyelectrolyte derivatives of poly(styrene-alt-maleic anhydride) (PSMA) functionalized with amino acids of different hydropathy indices, with the aim of evaluating the effect of the chemical functionality of polyelectrolytes on SH-SY5Y neuroblastoma cell adhesion. Functionalizing PSMA derivatives with l-glutamine, l-methionine, and l-tyrosine yielded PSMA-Gln, PSMA-Met, and PSMA-Tyr polyelectrolytes, respectively. We first studied the adsorption behavior of PSMA functionalized with amino acids on silicon wafer surfaces modified with 3-aminopropyltriethoxysilane at pH 4.0 and 7.0 and at low and high ionic strengths. The highest rate of polyelectrolyte adsorption was at pH 4.0 and high ionic strength and was higher with the glutamine and tyrosine films. The advance contact angles (θA) of the polyelectrolyte surfaces showed a moderate effect of ionic strength and pH on polyelectrolyte film wettability, with PSMA-Tyr being slightly more hydrophobic. Atomic force microscopy images of the polyelectrolyte surfaces showed two types of morphology: the well-defined globular nanostructure of PSMA-Met and PSMA-Tyr and densely packed nanofibrous-like structure of PSMA-Gln. The highest level of ionic strength caused a slight decrease in the size of the nanostructure that formed the surface domains, which was reflected in the degree of surface roughness. Cell adhesion assays with the polyelectrolyte film showed that SH-SY5Y neuroblastoma cells cultured on PSMA-Met present a well-extended morphology characterized by a stellate shape, with five or more actin-rich thin processes, whereas SH-SY5Y cells that were seeded on PSMA-Gln and PSMA-Tyr have a round morphology, with fewer and shorter processes. These results indicate that it is possible to modulate the surface characteristics of polyelectrolyte films based on their chemical functionality and environmental parameters such as pH and ionic strength in order to evaluate their effect on cell adhesion. Thus, surfaces prepared from polyelectrolytes functionalized with amino acids are an attractive and simple platform for cell adhesion, which can be used in developing biomaterials with modulated surface properties. © 2019 American Chemical Society.
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.subjectamino acid
dc.subjectcell adhesion
dc.subjectfunctionalization
dc.subjectpolyelectrolytes
dc.subjectSH-SY5Y neuroblastoma
dc.subjectAmino acids
dc.subjectAtomic force microscopy
dc.subjectCell adhesion
dc.subjectCells
dc.subjectCrystal atomic structure
dc.subjectFilm preparation
dc.subjectMorphology
dc.subjectNanostructures
dc.subjectpH effects
dc.subjectPolyelectrolytes
dc.subjectProteins
dc.subjectSilicon wafers
dc.subjectStyrene
dc.subjectSurface roughness
dc.subject3-aminopropyltriethoxysilane
dc.subjectChemical functionality
dc.subjectEffect of ionic strength
dc.subjectEnvironmental parameter
dc.subjectFunctionalizations
dc.subjectNeuroblastomas
dc.subjectPolyelectrolyte adsorption
dc.subjectSurface characteristics
dc.subjectIonic strength
dc.subjectamino acid
dc.subjectmaleic acid derivative
dc.subjectnanomaterial
dc.subjectpoly(styrene-alt-maleic anhydride)
dc.subjectpolyelectrolyte
dc.subjectpolymer
dc.subjectpolystyrene derivative
dc.subjectcell adhesion
dc.subjectchemistry
dc.subjectdrug effect
dc.subjecthuman
dc.subjectinfrared spectroscopy
dc.subjectpH
dc.subjectscanning electron microscopy
dc.subjectsurface property
dc.subjecttumor cell line
dc.subjectultrastructure
dc.subjectwettability
dc.subjectAmino Acids
dc.subjectCell Adhesion
dc.subjectCell Line, Tumor
dc.subjectHumans
dc.subjectHydrogen-Ion Concentration
dc.subjectMaleates
dc.subjectMicroscopy, Electron, Scanning
dc.subjectNanostructures
dc.subjectPolyelectrolytes
dc.subjectPolymers
dc.subjectPolystyrenes
dc.subjectSpectroscopy, Fourier Transform Infrared
dc.subjectSurface Properties
dc.subjectWettability
dc.titleAmino Acid-Functionalized Polyelectrolyte Films as Bioactive Surfaces for Cell Adhesion
dc.typeArticle


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