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Genotypic diversity of Acanthamoeba strains isolated from Chilean patients with Acanthamoeba keratitis

dc.contributor.authorJercic M.I.
dc.contributor.authorAguayo C.
dc.contributor.authorSaldarriaga-Córdoba M.
dc.contributor.authorMuiño L.
dc.contributor.authorChenet S.M.
dc.contributor.authorLagos J.
dc.contributor.authorOsuna A.
dc.contributor.authorFernández J.
dc.date.accessioned2020-09-02T22:21:09Z
dc.date.available2020-09-02T22:21:09Z
dc.date.issued2019
dc.identifier10.1186/s13071-019-3302-5
dc.identifier.citation12, 1, -
dc.identifier.issn17563305
dc.identifier.urihttps://hdl.handle.net/20.500.12728/5015
dc.descriptionBackground: Acanthamoeba spp. are the causative agents of a severe keratitis occurring mainly in contact lens wearers. The genus comprises more than 24 species that are currently divided into 20 different genotypes (T1-T20) according to sequence variations in the 18S rRNA gene. The objective of this study was to identify the genotypes and sub-genotypes of Acanthamoeba isolates collected at the Parasitology Laboratory of the Public Health Institute of Chile, the only laboratory in the country where Acanthamoeba screening is performed. This is the first report of genotypic identification of clinical isolates of Acanthamoeba in Chile and one of the few in South America. Results: In this study, 114 Acanthamoeba isolates from 76 Acanthamoeba keratitis patients, obtained between 2005-2016, were genotyped. T4 was the predominant genotype; T2 and T11 genotypes, which are scarcely reported worldwide, were also identified in Chilean patients (one and two patients, respectively). This is the first report of T2 and T11 genotypes isolated from Acanthamoeba keratitis patients in South America. It is also the first report of the T2 genotype circulating in this continent. Analysis of the diagnostic fragment 3 region of the 18S rRNA gene showed 24 T4 variants, with a predominance of the sub-genotype T4/A, followed by T4/B, T4/G, T4/C and T4/D. Bayesian analysis revealed three groups among the T4 variants: two well supported groups that included 12 and 7 sub-genotypes, respectively, and a weakly supported group that included 5 sub-genotypes. Most of the predominant T4 sub-genotypes belonged to the same group, which included 71.3% of the patients, while some minority variants lied mainly in the other two clusters. Conclusions: T2, T4 and T11 genotypes were predominantly isolated from the Acanthamoeba keratitis patients in Chile. Chilean predominant T4 sub-genotypes, which have also been reported worldwide, formed a separate cluster of the minority T4 variants. This study provides useful information about the predominant genotypes and subgenotypes that would be useful in selecting suitable strains to develop immunological and/or molecular diagnostic assays in Chile. © 2019 The Author(s).
dc.language.isoen
dc.publisherBioMed Central Ltd.
dc.subjectAcanthamoeba keratitis
dc.subjectASA.S1
dc.subjectDF3
dc.subjectT11 genotype
dc.subjectT2 genotype
dc.subjectT4 genotype
dc.subjectRNA 18S
dc.subjectAcanthamoeba
dc.subjectAcanthamoeba keratitis
dc.subjectArticle
dc.subjectBayes theorem
dc.subjectChile
dc.subjectChilean
dc.subjectfemale
dc.subjectgenetic strain
dc.subjectgenetic variability
dc.subjectgenotype
dc.subjecthuman
dc.subjectisolation procedure
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmicrobial diversity
dc.subjectmicrobial identification
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectphylogeny
dc.subjectRNA gene
dc.subjectSouth America
dc.subjectunindexed sequence
dc.subjectAcanthamoeba keratitis
dc.subjectclassification
dc.subjectgenetic variation
dc.subjectgenetics
dc.subjectgenotype
dc.subjectisolation and purification
dc.subjectparasitology
dc.subjectAcanthamoeba
dc.subjectAcanthamoeba Keratitis
dc.subjectChile
dc.subjectFemale
dc.subjectGenetic Variation
dc.subjectGenotype
dc.subjectHumans
dc.subjectMale
dc.subjectPhylogeny
dc.titleGenotypic diversity of Acanthamoeba strains isolated from Chilean patients with Acanthamoeba keratitis
dc.typeArticle


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