Molecular Insights into FaEG1, a Strawberry Endoglucanase Enzyme Expressed during Strawberry Fruit Ripening

Jara K.; Castro R.I.; Ramos P.; Parra-Palma C.; Valenzuela-Riffo F.; Morales-Quintana L.

dc.contributor.author	Jara K.
dc.contributor.author	Castro R.I.
dc.contributor.author	Ramos P.
dc.contributor.author	Parra-Palma C.
dc.contributor.author	Valenzuela-Riffo F.
dc.contributor.author	Morales-Quintana L.
dc.date.accessioned	2020-09-02T22:20:42Z
dc.date.available	2020-09-02T22:20:42Z
dc.date.issued	2019
dc.identifier	10.3390/plants8060140
dc.identifier.citation	8, 6, -
dc.identifier.issn	22237747
dc.identifier.uri	https://hdl.handle.net/20.500.12728/4968
dc.description	The endo-μ-1,4-glucanases (EGs) that belong to the glycosyl hydrolase family 9 (GH9) have roles in cell wall synthesis, remodeling and degradation. Previous studies have suggested that EGs may play a key role in the ripening of different fruits including strawberries. In this study, we used reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assays to determine the transcript accumulation of an endo-μ-1,4-glucanase (FaEG1) during fruit development in two different strawberry ‘Camarosa’ and ‘Monterey’ with contrasting softening ratios. Phylogenetic analyses suggest that FaEG1 belongs to the α group of the GH9 family with other proteins previously described with roles in elongation, abscission and ripening. Comparative modeling was used to obtain the FaEG1 structure. The model displays a α-barrel- type structure that is typical of the GH9 enzyme family, and comprises 12 α-helices, 2 310 helices and 6 μ-sheets. The catalytic residues were oriented to the solvent in the middle of an open groove. Protein-ligand interactions were explored with cellulose and two xyloglucans as ligands; the results suggest that the FaEG1-cellulose and FaEG1-XXXGXXXG (the most abundant xyloglucan in strawberries) complexes were more stable complexes than XXFGXXFG. The cell wall degradation was observed by scanning electron microscopy (SEM). The data are congruent with the probable role of the FaEG1 protein in the dissembly of the cellulose-hemicellulose fraction during the ripening of strawberry fruit. © 2019 by the authors. All rights reserved.
dc.language.iso	en
dc.publisher	MDPI AG
dc.subject	cell wall disassembly
dc.subject	endoglucanase
dc.subject	molecular dynamic simulations
dc.subject	molecular modeling
dc.subject	strawberry
dc.title	Molecular Insights into FaEG1, a Strawberry Endoglucanase Enzyme Expressed during Strawberry Fruit Ripening
dc.type	Article

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Molecular Insights into FaEG1, a Strawberry Endoglucanase Enzyme Expressed during Strawberry Fruit Ripening

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