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Effect of a ropy Exopolysaccharide-producing Bifidobacterium animalis subsp. Lactis strain orally administered on dss-induced colitis mice model

dc.contributor.authorHidalgo-Cantabrana C.
dc.contributor.authorAlgieri F.
dc.contributor.authorRodriguez-Nogales A.
dc.contributor.authorVezza T.
dc.contributor.authorMartínez-Camblor P.
dc.contributor.authorMargolles A.
dc.contributor.authorRuas-Madiedo P.
dc.contributor.authorGálvez J.
dc.date.accessioned2020-09-02T22:20:30Z
dc.date.available2020-09-02T22:20:30Z
dc.date.issued2016
dc.identifier10.3389/fmicb.2016.00868
dc.identifier.citation7, JUN, -
dc.identifier.issn1664302X
dc.identifier.urihttps://hdl.handle.net/20.500.12728/4897
dc.descriptionExopolysaccharide (EPS)-producing bifidobacteria, particularly Bifidobacterium animalis subsp. lactis strains, are used in the functional food industry as promising probiotics with purported beneficial effects. We used three isogenic strains of B. animalis subsp. lactis, with different EPS producing phenotypes (mucoid-ropy and non-ropy), in order to determine their capability to survive the murine gastrointestinal tract transit, as well as to evaluate their role in improving clinical outcomes in a chemically-induced colitis model. The three strains were able to survive in the intestinal tract of C57BL/6J mice during the course of the intervention study. Furthermore, the disease activity index (DAI) of the animal group treated with the ropy strain was significantly lower than of the DAI of the placebo group at the end of the treatment. However, no significant differences were found among the three strains. The analysis of several immune parameters, such as TNFα and IL-10 quantified in blood plasma and lymphocyte populations enumerated in mesenteric nodes, showed some significant variations among the four experimental animal groups. Remarkably, a higher capability of the ropy strain to increase regulatory T-cells in mesenteric lymphoid nodes was demonstrated, suggesting a higher ability of this strain to regulate inflammatory responses at mucosal level. Our data indicate that strains of B. animalis subsp. lactis producing EPS that confer a mucoid-ropy phenotype could represent promising candidates to perform further studies targeting intestinal inflammatory processes. © 2016 Hidalgo-Cantabrana, Algieri, Rodriguez-Nogales, Vezza, Martínez-Camblor, Margolles, Ruas-Madiedo and Gálvez.
dc.language.isoen
dc.publisherFrontiers Media S.A.
dc.subjectBifidobacterium
dc.subjectDSS-colitis
dc.subjectExopolysaccharide
dc.subjectImmune modulation
dc.subjectMouse model
dc.subjectMucoid
dc.subjectRopy
dc.subjectexopolysaccharide
dc.subjectinterleukin 10
dc.subjecttumor necrosis factor alpha
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectbacterial survival
dc.subjectBifidobacterium animalis
dc.subjectClinical Disease Activity Index
dc.subjectcontrolled study
dc.subjectdextran sodium sulfate-induced colitis
dc.subjectdisease assessment
dc.subjectenzyme linked immunosorbent assay
dc.subjectfeces analysis
dc.subjectfreeze drying
dc.subjectgene expression
dc.subjectgene sequence
dc.subjectimmune response
dc.subjectmale
dc.subjectmouse
dc.subjectnonhuman
dc.subjectreal time polymerase chain reaction
dc.subjectregulatory T lymphocyte
dc.subjectreverse transcription polymerase chain reaction
dc.titleEffect of a ropy Exopolysaccharide-producing Bifidobacterium animalis subsp. Lactis strain orally administered on dss-induced colitis mice model
dc.typeArticle


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